[Histonet] Muscle ATPase: Barbital Substitute

[Michele French]

Good Morning Everyone!
    I searched the archives and saw a few messages back in 2004 about 
using Glycine as a substitute for Sodium Barbital in the ATPase Stain 
for muscle fiber typing. The original e-mail did not include a recipe 
for the replacement of the barbital acetate preincubation solutions. It 
only talked about one preincubation solution at pH 9.4. I was wondering 
if anyone currently uses this substitute successfully and could send me 
your procedure?
Thanks!

Michele French
Bristol-Myers Squibb
Lawreceville, NJ