[Histonet] Why tissue sections from resin blocks have black deposits?

[Sulekha Ravi]

Dear members
  I have a problem with my resin blocks.We make resin blocks for non decalcified bone and vessel with metallic stent in my research laboratory. We dehydrate the tissue in ascending grades of alcohol, impregnate with methyl methacrylate monomer and then make blocks in polymerised methyl methacrylate.Some areas of tissue in these blocks have white opaque precipitate like dirt in it. When sections from such blocks are seen under microscope, these areas are black precipitate like deposits, masking the entire cellular architecture. We tried sonicating the sections in water with and without detergents. But nothing helped. Please help me to rectify this serious problem.
  Thanks
  Sulekha.
  Sr. Technical Asst
  SCTIMST
  Trivandrum
  Kerala
  India 695012

 		
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