[Histonet] pascal

Liz Chlipala liz <@t> premierlab.com
Wed Aug 16 13:18:41 CDT 2006



We use the pascal for all of our antibodies that require retrieval.  Since
we are in Colorado and at a higher elevation the highest temp we can get our
retrieval solution in the steamer is about 93 to 94+ degrees.  We still use
our steamer if we have problems with tissues falling off, buts its more of
an ordeal to make sure we are at the correct temp before we start.  It takes
over an hour for us to retrieve in the steamer since we monitor the
temperature of the retrieval solution the entire time.  We wait until the
solution gets to 93 degrees before we place the slides in it and then
continue to monitor, you will see a drop in temp once the slides are placed
in the solution and then we wait until the solution is back up to 93 degrees
and then we start the 20 minute retrieval process.  During this entire
process you need to also make sure that you don't run out of water, so we
definitely like the pascal and use it at the factory settings with good




Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC


Premier Laboratory, LLC

P.O. Box 18592

Boulder, CO 80308

phone (303) 735-5001

fax (303) 735-3540

liz <@t> premierlab.com



Ship to Address:


Premier Laboratory, LLC

University of Colorado at Boulder

MCDB, Room A3B40

Boulder, CO 80309


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