[Histonet] SurgiPath Paraffin

Gayle Callis gcallis <@t> montana.edu
Tue Aug 15 13:49:57 CDT 2006

Not sure what you mean by grit?  Do you stir your paraffin BEFORE 
embedding?  You may have the plastic polymers settling out to bottom of 
container.  if you pour pre melted paraffin into your processor paraffin 
containers,  give it a stir too.  Redistribution of polymers ensures the 
proper mixture for good sectioning.  We used this excellent paraffin system 
(infiltration media  then embedding medias) for years without any problems.

At 11:47 AM 8/15/2006, you wrote:
>Hi all,
>   I use the SurgiPath infiltration paraffin and the embedding paraffin in 
> my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding 
> station) as well as the stations on the tx processor.  It's not lot# 
> specific....I'm changing all paraffin stations on the processor every 
> Friday as well as emptying out the embedding chamber (cleaning with 
> xylene and 100% alcohol, as well as the filter) and by Monday, the grit 
> is there again. Anyone out there having the same problem???Any help would 
> be appreciated...
>Kim Tournear, HT (ASCP), QIHC (ASCP)
>Specialists in Dermatology
>Tucson, AZ
>Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls.  Great 
>rates starting at 1¢/min.
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>Histonet <@t> lists.utsouthwestern.edu

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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