[Histonet] autostainers & TMA's

Thom Jensen tissuearray <@t> hotmail.com
Mon Aug 14 11:21:04 CDT 2006

I would not recommend the Tape Transfer System for paraffin blocks.  The 
sections are not near the quality of regular micronomy (ribboning and using 
a water bath).  We have stained hundreds of TMA slides on our Dako and 
Vantana systems and have not had a problem with cores falling off.  If you 
use plus slides you should not have problems.  Just make sure the slides are 
dried well.

Thom Jensen

For information on problems with the Tape Sectioning Method go to:  

>From: "Helen Fedor" <hfedor <@t> jhmi.edu>
>To: <histonet <@t> lists.utsouthwestern.edu>,<GAshton <@t> PICR.man.ac.uk>
>Subject: Re: [Histonet] autostainers & TMA's
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>Sorry, for the last sentence.
>It should be: We do not experience tissue loss with this method.
> >>> Garry Ashton <GAshton <@t> PICR.man.ac.uk> 8/12/2006 8:38 AM >>>
>Dear histonetters
>I have 2 topics up for discussion.
>Firstly immunostainers.
>We are a small research lab and hence I'm interested in a machine that
>is totally flexible with regards to running several antibodies with 2
>different methods.
>Ideally an "open system" that will  also dewax & retrieve if possible.
>We've looked at dako and biogenix which are both open  systems and
>ventana and vision biosytems.
>Any comments on the above, or on any other systems available would be
>I know this subject ha been covered several times on the histonet, but
>an up to date view would be much appreciated.
>Secondly has anybody had any problems with regards to TMA sections
>falling off slides.
>We use 1mm cores and only put about 100 in each block, which are then
>cut and placed on APES coated slides.
>Sometimes up to 30% of the core may come off.
>I think next we'll try charged slides. Does anybody have any opinions
>ideas on this
>Many thanks in advance for any opinions.
>Garry Ashton
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