[Histonet] autostainers & TMA's

[Patsy Ruegg]

Because these sections are so precious, we use the tape transfer system for
them, the sections are cut using a tape which is then rolled onto to polymer
coated slides, the slides are exposed to UV causing the section to
polymerize to the slide allowing the tape to be removed.  This is the
Instrumedics section aide system which was just bought by McCormick.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Monday, August 14, 2006 6:53 AM
To: histonet <@t> lists.utsouthwestern.edu; GAshton <@t> PICR.man.ac.uk
Subject: Re: [Histonet] autostainers & TMA's

Sorry, for the last sentence.
It should be: We do not experience tissue loss with this method.
Helen

>>> Garry Ashton <GAshton <@t> PICR.man.ac.uk> 8/12/2006 8:38 AM >>>
Dear histonetters
I have 2 topics up for discussion.
 
Firstly immunostainers. 
We are a small research lab and hence I'm interested in a machine that is
totally flexible with regards to running several antibodies with 2
/3
different methods.
Ideally an "open system" that will  also dewax & retrieve if possible.
We've looked at dako and biogenix which are both open  systems and ventana
and vision biosytems.
Any comments on the above, or on any other systems available would be great.
I know this subject ha been covered several times on the histonet, but an up
to date view would be much appreciated.
 
Secondly has anybody had any problems with regards to TMA sections falling
off slides.
We use 1mm cores and only put about 100 in each block, which are then cut
and placed on APES coated slides.
Sometimes up to 30% of the core may come off.
I think next we'll try charged slides. Does anybody have any opinions /
ideas on this
 
Many thanks in advance for any opinions.
 
 
 
 
 
Garry Ashton
PICR
UK
 
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