[Histonet] liver albumin

[Geoff McAuliffe]

Hi Steve:

    Albumin is a very large protein that is largely confined to the 
vascular system. Yes, it is made in hepatocytes but I suspect that it 
diffuses quickly in sections. Also, the form that exists inside cells 
may not react with your marker? I suspect that freeze drying or freeze 
substitution might (emphasis on might) be the way to go here. Also, a 
PubMed or Medline search will reach a much larger database than Histonet 
and you might find the methods you seek.

Geoff


Steven Coakley wrote:

>Good afternoon,
>  We are attempting to stain cyrosections of mouse liver for albumin.  We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS.  Vascular stains but otherwise nothing.  Is anyone doing anything like this?
>   
>  Thanks,
>   
>  Steve
>
> 		
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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