[Histonet] liver albumin

Geoff McAuliffe mcauliff <@t> umdnj.edu
Fri Aug 11 15:05:57 CDT 2006

Hi Steve:

    Albumin is a very large protein that is largely confined to the 
vascular system. Yes, it is made in hepatocytes but I suspect that it 
diffuses quickly in sections. Also, the form that exists inside cells 
may not react with your marker? I suspect that freeze drying or freeze 
substitution might (emphasis on might) be the way to go here. Also, a 
PubMed or Medline search will reach a much larger database than Histonet 
and you might find the methods you seek.


Steven Coakley wrote:

>Good afternoon,
>  We are attempting to stain cyrosections of mouse liver for albumin.  We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS.  Vascular stains but otherwise nothing.  Is anyone doing anything like this?
>  Thanks,
>  Steve
>Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls.  Great rates starting at 1¢/min.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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