[Histonet] A different way to cut??

Lori Richey lrichey <@t> u.washington.edu
Fri Aug 11 12:24:20 CDT 2006

I've heard of that technique when trying to cut dried out and bloody 
specimens or dry  lymph nodes.  Not necessarily a 42 degree temp, but 
warming the block in the water bath first, and then back on the ice.  
The "freeze it" is optional and not necessary.

Brandi Farris wrote:

>I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle.
>Thank you,
>Brandi Farris
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

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