[Histonet] ASCP salary survey is deficient

Morken, Tim - Labvision tpmorken <@t> labvision.com
Thu Aug 3 10:41:15 CDT 2006


It seems the ASCP survey is grossly deficient .The recently published survey
has woefully incomplete data for the majority of the country.
It is so incomplete that they don't even show salary data for lthe majority
of the US. :

HT only: IL, IN, MI, OH, WI, AR, LA, OK, TX 
No HT: IA, KS, MN, MO, NE, ND, SD, AK, AZ, CA, CO, HI, ID, MT, NV, NM, OR,
UT, WA, WY
NO DATA AT ALL!!: AL, DE, CD, FL, GA, KY, MD, MS, NC, SC, TN, VA, WV.

In fact, only the North East ( CT, ME, MA, NH, NJ, NY, PS, RI, VT ) has
complete data for HT, HTL and HT supervisors.

And it is like this for every lab tech group. 

This doesn't seem like much of a survey to me, despite their claims they
made every effort to get data.  They got 749 out of 3,544 eligible labs, but
obvioulsy those responding were highly concentrated in only  a few areas. As
such, this survey is nearly useless to most ASCP members.

The Advance agazine survey mentioned below is a very different sort of
survey - individuals self-responding to a magazine survey. I'm not sure it
is much more accurate due to that bias, but it does give very different
results.

Tim Morken




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LuAnn
Anderson
Sent: Thursday, August 03, 2006 8:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mohs Tech salaries


Wow--this survey is way lower than the one Advance conducted last 
year!! It was published in the 12/7/2005 issue and can be found 
online. It seems much more realistic, given the current circumstances 
in the field. The ASCP survey  always seems to be on the low side for 
salaries. I have always questioned who they send the surveys out 
to--but have not gotten an answer on that as of yet!!



At 09:17 PM 8/2/2006, you wrote:
>Not specifically for Mohs, but I thought I would let Histonetters know 
>that the 2005 ASCP Wage and Vacancy survey results are now on the ASCP 
>BOR
>webpage:
>
>http://www.ascp.org/Certification/ForProgramDirectors/research/default.
>aspx
>
>Click on 2005.
>
>Survey done Nov. 2005, will be published in the August 2006 "Laboratory 
>Medicine". Found it on their website last week.
>
>Peggy A. Wenk, HTL(ASCP)SLS
>William Beaumont Hospital
>Royal Oak, MI 48073
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
>nsdd114 <@t> alltel.net
>Sent: Wednesday, August 02, 2006 7:27 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Mohs Tech salaries
>
>I was wondering if those of you who are doing Mohs would respond to my 
>question.  I am HT-ASCP certified and have 15 years in the field.  I am 
>currently doing Mohs, because the hours and the pay are better in Mohs 
>in my area.  I like doing Mohs and we keep very busy.  We started the 
>lab a year ago and have one Mohs surgeon and are currently doing about 
>1500 cases a year.  Of course that is cases not blocks, because we 
>frequently do cases with anywhere from 6-25 pieces.  Our practice would 
>like to expand our service and we have tried three techs, all on the 
>job trained and not certified.  I personally know techs who were OJT 
>trained who are top notch techs.  Two of the techs we interviewed 
>weren't sufficiently skilled to work really independently at high 
>volume and trouble shoot routines.  One didn't know how to tell if she 
>had a complete skin edge under the scope and she had 3 years of 
>experience.  That's scarey.  The 3rd tech is pretty capable, but is 
>also a CMA and prefers the nursing position.  My question is "Does 
>anyone know of a Mohs salary survey or any guidelines as far as pay 
>goes for both certified and non-certified techs?"  I personally don't 
>care if someone is confident and capable whether they are certified or 
>not since it isn't required at this time, but we do want to establish a 
>pay scale that rewards people based on their overall training and 
>skill.  I do suspect that the next few years will bring some kind of 
>standardized training requirements and/or certification for Mohs techs.  
>If anyone has any input I'd appreciate it.  We are in the south central
east coast area. Thanks, Nancy
> >
> > From: histonet-request <@t> lists.utsouthwestern.edu
> > Date: 2006/08/02 Wed PM 12:01:20 CDT
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: Histonet Digest, Vol 33, Issue 2
> >
> > Send Histonet mailing list submissions to
> >       histonet <@t> lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> >       http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body 'help' to
> >       histonet-request <@t> lists.utsouthwestern.edu
> >
> > You can reach the person managing the list at
> >       histonet-owner <@t> lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific 
> > than "Re: Contents of Histonet digest..."
> >
> >
> > Today's Topics:
> >
> >    1. RE: Foxp3 on Murine tissue (Melissa Gonzalez)
> >    2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk))
> >    3. Staining cultured cells (Pixley, Sarah (pixleysk))
> >    4. immunohistochemical analysis for connective tissue (Xilong Li)
> >    5. ImmunoVision Technologies, Co. (Helen E Johnson)
> >    6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist)
> >    7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum)
> >    8. RE: Ethanol/other fixations for frozen sections (Tony Henwood)
> >    9. Looking for Florida histotechs--or people who are       interested
> >       in Florida relocation: we help with the license. (Cheryl)
> >   10. article from J of histotechnology (louise renton)
> >   11. Zinc-Based Fixative (Shirley PHUA)
> >   12. prefilled formalin containers (Vickroy, Jim)
> >   13. Re: prefilled formalin containers (Jackie M O'Connor)
> >   14. '07 TSH Meeting (Ford Royer)
> >   15. The New VIP (Santana, Diane)
> >   16. Re: The New VIP (Fred Underwood)
> >   17. RE: The New VIP.    . (Henry, Charlene)
> >   18. Re: The New VIP (crains <@t> wpmpath.com)
> >   19. prefilled formalin containers  (Stahl, Michael)
> >   20. Re: The New VIP (Andrea Grantham)
> >   21. charging and CPT questions (Vickroy, Jim)
> >   22. please unsubscribe me from the list (Jayne Scoggin)
> >   23. RE: charging and CPT questions (Zajic Kari)
> >
> >
> > --------------------------------------------------------------------
> > --
> >
> > Message: 1
> > Date: Tue, 1 Aug 2006 10:18:52 -0700
> > From: "Melissa Gonzalez" <Melissa.Gonzalez <@t> cellgenesys.com>
> > Subject: [Histonet] RE: Foxp3 on Murine tissue
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <A6B0506C2B000E45A7556B9A93C455475CAE3B <@t> hqsvr01mail.cgi.com>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> > I've also tried several of the products out there with no success on 
> > murine tissues as well. I would be interested in hearing if anyone 
> > has had success with FoxP3 yet on murine tissues, and what you could 
> > use as a positive control.
> >
> > Thanks,
> > Melissa
> > ------------------------------
> >
> > Message: 7
> > Date: Mon, 31 Jul 2006 14:02:03 -0500
> > From: "Drew Allan Roenneburg" <ROENN <@t> surgery.wisc.edu>
> > Subject: [Histonet] Foxp3 on Murine tissue
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <44CE0D5B0200009C00001F23 <@t> gw.surgery.wisc.edu>
> > Content-Type: text/plain; charset=US-ASCII
> >
> > Hi, I was wondering if anyone has stained for foxp3 on frozen or 
> > FFPE mouse tissues (spleen).  I have tried the Abcam rabbit 
> > anti-human foxp3 antibody that works on FFPE human and monkey 
> > (tonsil and spleen), however have had little success on mouse.  
> > Thanks Drew Roenneburg
> >
> >
> >
> > ------------------------------
> >
> > Message: 2
> > Date: Tue, 1 Aug 2006 13:45:30 -0400
> > From: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
> > Subject: [Histonet] RE:BrdU and GFP staining
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <E7340445E0595A418B2F8DE0AE480E2007A35A42 <@t> ucmail6.ad.uc.edu>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> >  Dear Steve:
> > I would stain for GFP with an antibody to GFP and do labeling with 
> > DAB. I recommend the Elite ABC kits. The DAB is moderately resistant 
> > to the BrdU penetration protocols. Then, fix your tissues well with 
> > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using 
> > a system that labels with a fluorescent antibody. This will only 
> > work, however, if your GFP is in different cells, or it is in the 
> > cytoplasm of large cells that have no nuclear staining. The DAB can 
> > obscure the secondary fluorescent labeling.
> >
> > Good luck. Stay Cool back at you.
> > Sarah Pixley,
> > Ohio
> >
> > Message: 2
> > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT)
> > From: Steven Coakley <sjchtascp <@t> yahoo.com>
> > Subject: [Histonet] BrdU and GFP expression
> > To: Histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <20060731172241.65699.qmail <@t> web38215.mail.mud.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Good afternoon from WI,
> >
> >   We have several FFPE blocks from 2003 that express GFP.  Our lead 
> > scientist would like to perform Brdu IHC in the same slides hoping 
> > to express both.  As expected the AR removes the GFP green label.  
> > Has anyone any experience expressing Brdu and GFP on the same slide.
> >
> >   Stay cool,
> >
> >   Steve
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 3
> > Date: Tue, 1 Aug 2006 13:49:31 -0400
> > From: "Pixley, Sarah \(pixleysk\)" <PIXLEYSK <@t> UCMAIL.UC.EDU>
> > Subject: [Histonet] Staining cultured cells
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <E7340445E0595A418B2F8DE0AE480E2007A35A43 <@t> ucmail6.ad.uc.edu>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> >
> > To stain cultured cells we routinely fix with 4% paraformaldehyde 
> > for 15-20 mins. But if the antigen is sensitive to aldehydes, then 
> > we fix with ice-cold 100% methanol for 5-10 mins. Your acetone 
> > technique for 30 mins is extremely harsh for cultured cells. If you 
> > want to continue with it, I would fix with the acetone for only a 
> > few minutes and try that.
> >
> > As for the other person who asked about staining cultured cells, we 
> > do exactly the same ICC as on sections, although we usually are able 
> > to dilute the primary antibodies more than with frozen tissue 
> > sections (using paraformaldehyde perfused tissues).
> >
> > Sarah Pixley
> > Ohio
> >
> >
> > Message: 4
> > Date: Mon, 31 Jul 2006 13:41:54 -0400
> > From: Christopher C Overend 
> > <christopher.overend <@t> huskymail.uconn.edu>
> > Subject: [Histonet] IFA staining problems
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90 <@t> huskymail.uconn.edu>
> > Content-Type: text/plain; charset=us-ascii
> >
> > I am new to the technique of immunofluorescence, and have been doing 
> > some work with it lately on cultured cells. Specifically, I have 
> > been staining intracellular virus with very good results. However, 
> > when i try to detect cellular proteins, I do not get any staining. 
> > The proceedure i have been using consists of "fixation" with 90% 
> > acetone for 30 min, at 4degrees C. the rest of the process had been 
> > much like an ELISA, all incubations have been for 30 min at 
> > 4degrees. The buffer i have been using is PBS, 1%BSA, 0.1%NaN3. 
> > Someone mentioned the problem could be from the acetone and 
> > suggested trying a glyoxal fixative. Can anyone offer some insights, 
> > or if there is a different protocol i might have to follow using a 
> > glyoxal fixative with tissue
>culture?
> > Thank you!
> > Chris
> >
> > Christopher Overend
> > Ph.D Student
> > University of Connecticut
> > Department of Pathobiology and Veterinary Sciences Storrs, CT 
> > Christopher.overend <@t> uconn.edu
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 4
> > Date: Tue, 01 Aug 2006 13:23:06 -0500
> > From: "Xilong Li" <Xilong.Li <@t> UTSouthwestern.edu>
> > Subject: [Histonet] immunohistochemical analysis for connective 
> > tissue
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <44CF55BA020000F000004E06 <@t> swnw124.swmed.edu>
> > Content-Type: text/plain; charset=US-ASCII
> >
> > Hi, All members,
> >
> > I am looking for a consistent protocol to stain collagen of 
> > connective tissues in muscle frozen sections by immunohistochemical 
> > analysis including information for primary antibody and second 
> > antibody used in the protocols.
> >
> > Thanks in advance.
> >
> > xilong li
> >
> > Dr. Xilong Li
> > Hypertension Division, Internal Medicine University of Texas 
> > Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142
> > Dallas, TX 75390
> >
> > Tel: 214-648-9966(L)
> > Fax: 214-648-7902
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 5
> > Date: Tue, 1 Aug 2006 14:24:37 -0400
> > From: Helen E Johnson <hej01 <@t> health.state.ny.us>
> > Subject: [Histonet] ImmunoVision Technologies, Co.
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID:
> >
> > <OFD5CF0F47.E75933CF-ON852571BD.0064AF32-852571BD.0065230A <@t> notes.hea
> > lt
> > h.state.ny.us>
> >
> > Content-Type: text/plain; charset=US-ASCII
> >
> >
> > Hi Histonetters,
> >       Does anyone know if ImmunoVision Technologies, Co. is still in 
> > business?  No one answers the phone ( 650-992-7563).
> >                                                          Helen
> > (hej01 <@t> health.state.ny.us)
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 6
> > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT)
> > From: Ron Lagerquist <spinquist <@t> yahoo.com>
> > Subject: Re: [Histonet] ImmunoVision Technologies, Co.
> > To: Helen E Johnson <hej01 <@t> health.state.ny.us>,
> >       histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <20060801183241.80617.qmail <@t> web56106.mail.re3.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > They were purchased by VisionBiosystems (800-753-7264)
> >   http://www.vision-bio.com/media_releases.html
> >
> >   Ron
> >
> > Helen E Johnson <hej01 <@t> health.state.ny.us> wrote:
> >
> > Hi Histonetters,
> > Does anyone know if ImmunoVision Technologies, Co. is still in 
> > business? No one answers the phone ( 650-992-7563). Helen
> > (hej01 <@t> health.state.ny.us)
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ---------------------------------
> > Do you Yahoo!?
> >  Next-gen email? Have it all with the  all-new Yahoo! Mail Beta.
> >
> > ------------------------------
> >
> > Message: 7
> > Date: Tue, 01 Aug 2006 14:33:51 -0400
> > From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
> > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID: <6.1.1.1.2.20060801142646.01a26d88 <@t> mail.vet.upenn.edu>
> > Content-Type: text/plain; charset="us-ascii"; format=flowed
> >
> >
> >
> > Good Afternoon All,
> >
> > We have our web site up and partially up.  We do have our program 
> > online and have the program in the download file area for you to 
> > review and if register.  The program can be printed off.  Just go to 
> > www.pahisto.org and look for downloads in the top line.  Click on 
> > and open or save.  The format is a Microsoft Word document.  If you 
> > have a problem or would need a pre-printing copy please let either 
> > me or Gloria Limetti know.  Gloria can be reached at 
> > glorialimetti <@t> yahoo.com or
>412-647-8535.
> >
> > Thanks for your patience and we will be updating the program with 
> > vendors and other information as we near the meeting in October.
> >
> >
> > Best Regards,
> >
> > Pamela A Marcum
> > Manager, Histology Special Procedures
> > University of Pennsylvania
> > School of Veterinary Medicine
> > R.S. Reynolds Jr.  CORL
> > New Bolton Center
> > 382 West Street Road
> > Kennett Square, PA 19348
> >
> > Phone - 610-925-6278
> > Fax     - 610-925-8120
> > E-mail - pmarcum <@t> vet.upenn.edu
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 8
> > Date: Wed, 2 Aug 2006 10:49:46 +1000
> > From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
> > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections
> > To: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>, "Histonet"
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: 
> > <B9EAF61856077F47BF9BE2F89AFC55520127C6FF <@t> hedwig.nch.kids>
> > Content-Type: text/plain; charset="us-ascii"
> >
> > Urgent Frozen Sections for H&E - we fix in methanol
> >
> > Regards
> >
> > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory 
> > Manager & Senior Scientist The Children's Hospital at Westmead, 
> > Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> > Tel: 612 9845 3306
> > Fax: 612 9845 3318
> >
> >
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> > Andrea T. Hooper
> > Sent: Monday, 31 July 2006 10:45 PM
> > To: Histonet
> > Subject: [Histonet] Ethanol/other fixations for frozen sections
> >
> >
> > Just taking a survey .... What are everyone's thoughts on using 
> > ethanol for frozen sections? I have a colleague who swears by it but 
> > I am an acetone person myself.
> >
> > Thoughts, comments, input on what is the best fix for frozen section 
> > IHC?
> >
> > Thanks!
> > --
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ********************************************************************
> > **
> > This email and any files transmitted with it are confidential and
intended
>solely for the use of the individual or entity to whom they are 
>addressed. If you are not the intended recipient, please delete it and 
>notify the sender.
> >
> > Views expressed in this message and any attachments are those of the 
> > individual sender, and are not necessarily the views of The 
> > Children's Hospital at Westmead
> >
> > This note also confirms that this email message has been virus 
> > scanned and although no computer viruses were detected, the 
> > Childrens Hospital at
>Westmead accepts no liability for any consequential damage resulting 
>from email containing computer viruses.
> > ********************************************************************
> > **
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 9
> > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT)
> > From: Cheryl <tkngflght <@t> yahoo.com>
> > Subject: [Histonet] Looking for Florida histotechs--or people who are
> >       interested in Florida relocation: we help with the license.
> > To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <20060802024034.14424.qmail <@t> web50904.mail.yahoo.com>
> > Content-Type: text/plain; charset=iso-8859-1
> >
> > Hello everyone--
> >
> >   Florida's registry process has created a major shortage of 
> > qualified
>techs in the state.  We have a number of Florida permanent and temp 
>opportunities.  The permanent labs will consider transitional job 
>situations to allow you to work while your license is processed.  We 
>also have a number of temp situations for a great pay scale for techs 
>registered to work in the state. Travel isn't for everyone, but these 
>are good labs and a great way to start.
> >
> >   Either way, we'll help you through the process to get you through 
> > it as
>quickly as possible.  If you're even curious, give us a call.  Of 
>course we have a number of other opportunities including sales and 
>management all over the country.  We won't work with every lab--those 
>we place find themselves in jobs that fit--where they are happy to go 
>to work.  We're here to help and no fee to you.
> >
> >   Tiffany is in the office and I'm traveling as a temp for a couple 
> > of
>weeks--as always, referral bonuses are available--share us with your 
>friends!
> >
> >   Tiffany/Office 281.852.9457
> >   Cheryl/Cell 281.883.7704
> >   or this number follows me around: 800.756.3309 (it will ring a 
> > long time to find me--please be patient and leave your phone 
> > number!)
> >
> >   We return all calls.
> >
> >   Thanks!
> >
> >   Cheryl
> >
> >
> >
> > Cheryl Kerry, HT(ASCP)
> > Full Staff Inc.
> > Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c
> > 281.852.9457 o
> > admin <@t> fullstaff.org
> >
> > Sign up for the FREE monthly newsletter AP News--updates, tricks of 
> > the
>trade and current issues for Anatomic Pathology Clinical Labs. Send a 
>'subscribe' request to APNews <@t> fullstaff.org. Please include your name 
>and specialty in the body of the email.
> >
> > ------------------------------
> >
> > Message: 10
> > Date: Wed, 2 Aug 2006 09:47:06 +0200
> > From: "louise renton" <louise.renton <@t> gmail.com>
> > Subject: [Histonet] article from J of histotechnology
> > To: Histonet <@t> lists.utsouthwestern.edu
> > Message-ID:
> >       <e483362e0608020047v2e62c7b9u9a6284948826acae <@t> mail.gmail.com>
> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> >
> > Hi can anyone share a copy of this article? I would be very grateful
> >
> > "Entering the realm of mineralized bone processing: a review of the 
> > literature and techniques." J Histotechnol 1997:20 (3) p259-266
> >
> > Many thanks
> >
> > Louise Renton
> > Bone Research Unit
> > University of the Witwatersrand
> > Johannesburg
> > South Africa
> > "There are nights when the wolves are silent and only the moon 
> > howls". George Carlin No trees were killed in the sending of this 
> > message. However, many electrons were terribly inconvenienced.
> >
> >
> >
> > ------------------------------
> >
> > Message: 11
> > Date: Wed, 2 Aug 2006 16:19:02 +0800
> > From: Shirley PHUA <Shirley_PHUA <@t> hsa.gov.sg>
> > Subject: [Histonet] Zinc-Based Fixative
> > To: histonet <@t> lists.utsouthwestern.edu
> > Message-ID:
> >
> > <OF3FBA6707.3B4F8EC6-ON482571BE.002C8BD9-482571BE.002DAEB0 <@t> gems2.gov
> > .s
> > g>
> >
> > Content-Type: text/plain; charset="US-ASCII"
> >
> > Dear All,
> >
> > Reference Article:
> > Lab Invest 2003 Jun;83(6):889-99
> > Zinc-based fixative improves preservation of genomic DNA and 
> > proteins in histoprocessing of human tissues
> >
> > Anyone out there know of the exact composition of any zinc-based 
> > fixative that is based on the following? 1. 0.5% Zinc Chloride and
> > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium
> > acetate
> >
> >
> > Many thanks & regards,
> > Shirley Phua
> > Histopathology Laboratory
> > Centre for Forensic Medicine
> > Health Sciences Authority
> > Singapore
> >
> >
> >
> > ------------------------------
> >
> > Message: 12
> > Date: Wed, 2 Aug 2006 08:23:30 -0500
> > From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> > Subject: [Histonet] prefilled formalin containers
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <A2AD4F58AD06B44FBA71D4B1C4B9571A0680A390 <@t> mmcmail.ad.mhsil.com>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> >
> >
> > We have experienced problems with prefilled formalin containers that 
> > we send to our clients.  We have tried two different vendors and 
> > they both leak.  One of the container types claims to have an o-ring 
> > type lid  and the other does not have an o-ring.  The containers 
> > with the o-ring seems to leak as much as the others.  In fact the 
> > o-ring type sometimes leak when they are received from the vendor.  
> > If anyone has dealt with this successfully please let me know how 
> > you solved it.
> >
> >
> >
> > Jim Vickroy
> >
> > Technical Supervisor - Surgical and Autopsy Pathology
> >
> > Memorial Medical Center
> >
> >
> >
> >
> >
> > This message (including any attachments) contains confidential 
> > information intended for a specific individual and purpose, and is 
> > protected by law. If you are not the intended recipient, you should 
> > delete this message. Any disclosure, copying, or distribution of 
> > this
>message, or the taking of any action based on it, is strictly 
>prohibited.
> >
> >
> > ------------------------------
> >
> > Message: 13
> > Date: Wed, 2 Aug 2006 08:41:32 -0500
> > From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
> > Subject: Re: [Histonet] prefilled formalin containers
> > To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> > Cc: histonet <@t> lists.utsouthwestern.edu,
> >       histonet-bounces <@t> lists.utsouthwestern.edu
> > Message-ID:
> >
> > <OFFB622EF1.746166AF-ON862571BE.004AF8E5-862571BE.004B3FD8 <@t> abbott.co
> > m>
> > Content-Type: text/plain; charset="US-ASCII"
> >
> > Are they leaking on the return trip?  Hard to get your clients to 
> > make sure they are screwed on properly.  Each vial/jar should be 
> > contained within a secondary leakproof bag, anyway - to prevent 
> > leaks in transit. Give Surgipath a call.
> >
> > Jackie O'
> >
> >
> >
> > "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com> Sent by: 
> > histonet-bounces <@t> lists.utsouthwestern.edu
> > 08/02/2006 08:23 AM
> >
> > To
> > <histonet <@t> lists.utsouthwestern.edu>
> > cc
> >
> > Subject
> > [Histonet] prefilled formalin containers
> >
> >
> >
> >
> >
> >
> >
> >
> > We have experienced problems with prefilled formalin containers that 
> > we send to our clients.  We have tried two different vendors and 
> > they both leak.  One of the container types claims to have an o-ring 
> > type lid  and the other does not have an o-ring.  The containers 
> > with the o-ring seems to leak as much as the others.  In fact the 
> > o-ring type sometimes leak when they are received from the vendor.  
> > If anyone has dealt with this successfully please let me know how 
> > you solved it.
> >
> >
> >
> > Jim Vickroy
> >
> > Technical Supervisor - Surgical and Autopsy Pathology
> >
> > Memorial Medical Center
> >
> >
> >
> >
> >
> > This message (including any attachments) contains confidential 
> > information intended for a specific individual and purpose, and is 
> > protected by law. If you are not the intended recipient, you should 
> > delete this message. Any disclosure, copying, or distribution of 
> > this message, or the taking of any action based on it, is strictly 
> > prohibited. _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 14
> > Date: Wed, 2 Aug 2006 08:42:33 -0500
> > From: "Ford Royer" <froyer <@t> bitstream.net>
> > Subject: [Histonet] '07 TSH Meeting
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <003101c6b639$82553090$7701a80a <@t> Ford>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> > Would the person(s) who are organizing next years Texas Society of 
> > Histotechnology meeting please contact me?
> >
> > Thanks!
> >
> > ~ Ford
> >
> > Ford M. Royer, MT(ASCP)
> > Histology Product Manager
> > Minnesota Medical, Inc.
> > 7177 Madison Ave. W.
> > Golden Valley, MN 55427-3601
> > CELL:  612-839-1046
> > Phone:  763-542-8725
> > Fax:  763-546-4830
> > eMail:  froyer <@t> bitstream.net
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 15
> > Date: Wed, 2 Aug 2006 10:05:40 -0400
> > From: "Santana, Diane" <dsantana <@t> pmaonline.com>
> > Subject: [Histonet] The New VIP
> > To: "'histonet <@t> lists.utsouthwestern.edu'"
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5 <@t> MAILPMA>
> > Content-Type: text/plain;     charset="iso-8859-1"
> >
> > I have a question and please nobody get all defensive about this, 
> > but has anybody recently bought the VIP 5 tissue processor? If you 
> > have, do or did you have any problems with it? Thanks
> > Diane Santana
> > PMA
> > Haverhill, Mass.
> >
> >
> >
> > ------------------------------
> >
> > Message: 16
> > Date: Wed, 02 Aug 2006 10:31:26 -0400
> > From: "Fred Underwood" <funderwood <@t> mcohio.org>
> > Subject: Re: [Histonet] The New VIP
> > To: <histonet <@t> lists.utsouthwestern.edu>, <dsantana <@t> pmaonline.com>
> > Message-ID: <s4d07f0a.084 <@t> mcohio.org>
> > Content-Type: text/plain; charset=US-ASCII
> >
> > I've had mine for a year and (knock, knock on wood) have had no 
> > problems.
> >
> > Fred
> >
> > >>> "Santana, Diane" <dsantana <@t> pmaonline.com> 08/02 10:05 AM >>>
> > I have a question and please nobody get all defensive about this, 
> > but has anybody recently bought the VIP 5 tissue processor? If you 
> > have, do or did
> > you have any problems with it?
> > Thanks
> > Diane Santana
> > PMA
> > Haverhill, Mass.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > Message: 17
> > Date: Wed, 2 Aug 2006 09:36:48 -0500
> > From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
> > Subject: RE: [Histonet] The New VIP.    .
> > To: "Santana, Diane" <dsantana <@t> pmaonline.com>,
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >
><5CB39BCA5724F349BCB748675C6CA1A2099A1D6A <@t> SJMEMXMB02.stjude.sjcrh.local
>>
> >
> > Content-Type: text/plain;     charset="US-ASCII"
> >
> > We purchased a VIP5 a few years ago and had several problems so it 
> > was replaced with a new VIP5. Since that time we have had no 
> > problems and it has been a reliable instrument. Charlene
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> > Santana, Diane
> > Sent: Wednesday, August 02, 2006 9:06 AM
> > To: 'histonet <@t> lists.utsouthwestern.edu'
> > Subject: [Histonet] The New VIP. .
> >
> > I have a question and please nobody get all defensive about this, 
> > but has anybody recently bought the VIP 5 tissue processor? If you 
> > have, do or did you have any problems with it? Thanks
> > Diane Santana
> > PMA
> > Haverhill, Mass.
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 18
> > Date: Wed, 2 Aug 2006 10:30:16 -0500
> > From: <crains <@t> wpmpath.com>
> > Subject: Re: [Histonet] The New VIP
> > To: "Santana, Diane" <dsantana <@t> pmaonline.com>,
> >       "'histonet <@t> lists.utsouthwestern.edu'"
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       
> > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com <@t> dukecmmtao03>
> > Content-Type: text/plain; charset=ISO-8859-1
> >
> > We have a VIP 5 that we purchased a little over a year ago.  Have 
> > not had
>a single problem with it so far.
> >
> > Chris Rains
> > WPM Pathology Laboratory
> > Salina, KS
> > >
> > > From: "Santana, Diane" <dsantana <@t> pmaonline.com>
> > > Date: 2006/08/02 Wed AM 09:05:40 CDT
> > > To: "'histonet <@t> lists.utsouthwestern.edu'"
><histonet <@t> lists.utsouthwestern.edu>
> > > Subject: [Histonet] The New VIP
> > >
> > > I have a question and please nobody get all defensive about this, 
> > > but
>has
> > > anybody recently bought the VIP 5 tissue processor? If you have, 
> > > do or
>did
> > > you have any problems with it?
> > > Thanks
> > > Diane Santana
> > > PMA
> > > Haverhill, Mass.
> > >
> > > _______________________________________________
> > > Histonet mailing list
> > > Histonet <@t> lists.utsouthwestern.edu 
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > >
> >
> > Important:  This email and any attachments may contain confidential
>information subject to protection under the Federal Standards for 
>Privacy of Individually Identifiable Health Information (45 C.F.R. 
>Parts 160 and 164). If you or your organization is a "Covered Entity" 
>under the above mentioned regulations, you are obligated to treat such 
>information in a manner consistent with the regulations.  If it appears 
>that this email was sent to you in error, (1) you are prohibited from 
>utilizing or disseminating this email or any attachments; (2) please 
>immediately delete it from your computer and any servers or other 
>locations where it might be stored and email (crains <@t> wpmpath.com) or 
>call (Chris Rains) at 785-823-7201 advising that you have done so.  We 
>appreciate your cooperation.
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 19
> > Date: Wed, 2 Aug 2006 11:42:17 -0400
> > From: "Stahl, Michael" <mstahl <@t> bvha.org>
> > Subject: [Histonet] prefilled formalin containers
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <4C878E714B21EB4F8EB159777B8822EE5B6834 <@t> bvfyms01.net.bvha.org>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> >  We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. 
> > We found these to be almost leak proof. However, they do leak when 
> > the jars  arnt screwed on correctly. But we agree, its not pleasant 
> > to have a specimen jar in a bio hazard bag full of fixative. Just 
> > try to find that cervial biospy!
> >
> > Michael P. Stahl HT (ASCP)
> > BVRHC
> > 145 W Wallace St
> > Findlay, OH 45840
> > mstahl <@t> bhva.org
> >
> >
> > ------------------------------
> >
> > Message: 20
> > Date: Wed, 02 Aug 2006 08:55:45 -0700
> > From: Andrea Grantham <algranth <@t> u.arizona.edu>
> > Subject: Re: [Histonet] The New VIP
> > To: "'histonet <@t> lists.utsouthwestern.edu'"
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       
> > <4.3.2.7.2.20060802085142.00cec460 <@t> algranth.inbox.email.arizona.edu>
> > Content-Type: text/plain; charset="us-ascii"; format=flowed
> >
> > I just got a new VIP 5 and I love it. Wanted one all my (histotech) 
> > life and I'm so happy to finally have it! It has been great. So easy 
> > to use and
>
> > the tissues are coming out wonderful. Knock-on-wood it will do as 
> > well as the old Tissue Tek dipper/dunker that it is replacing. Andi
> >
> >
> > At 10:30 AM 8/2/2006 -0500, crains <@t> wpmpath.com wrote:
> > >We have a VIP 5 that we purchased a little over a year ago.  Have 
> > >not had
>
> > >a single problem with it so far.
> > >
> > >Chris Rains
> > >WPM Pathology Laboratory
> > >Salina, KS
> > > >
> > > > From: "Santana, Diane" <dsantana <@t> pmaonline.com>
> > > > Date: 2006/08/02 Wed AM 09:05:40 CDT
> > > > To: "'histonet <@t> lists.utsouthwestern.edu'"
> > > <histonet <@t> lists.utsouthwestern.edu>
> > > > Subject: [Histonet] The New VIP
> > > >
> > > > I have a question and please nobody get all defensive about 
> > > > this, but
>has
> > > > anybody recently bought the VIP 5 tissue processor? If you have, 
> > > > do or
>did
> > > > you have any problems with it?
> > > > Thanks
> > > > Diane Santana
> > > > PMA
> > > > Haverhill, Mass.
> > > >
> > > > _______________________________________________
> > > > Histonet mailing list
> > > > Histonet <@t> lists.utsouthwestern.edu 
> > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > > >
> > >
> > >Important:  This email and any attachments may contain confidential 
> > >information subject to protection under the Federal Standards for 
> > >Privacy
>
> > >of Individually Identifiable Health Information (45 C.F.R. Parts 
> > >160 and 164).  If you or your organization is a "Covered Entity" 
> > >under the above mentioned regulations, you are obligated to treat 
> > >such information in a manner consistent with the regulations.  If 
> > >it appears that this email
>was
> > >sent to you in error, (1) you are prohibited from utilizing or 
> > >disseminating this email or any attachments; (2) please immediately
>delete
> > >it from your computer and any servers or other locations where it 
> > >might
>be
> > >stored and email (crains <@t> wpmpath.com) or call (Chris Rains) at 
> > >785-823-7201 advising that you have done so.  We appreciate your
>cooperation.
> > >
> > >
> > >_______________________________________________
> > >Histonet mailing list
> > >Histonet <@t> lists.utsouthwestern.edu
> > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > .....................................................................
> > : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> > : Sr. Research Specialist       University of Arizona               :
> > : (office:  AHSC 4212)          P.O. Box 245044                     :
> > : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> > : (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
> > :...................................................................:
> >            http://www.cba.arizona.edu/histology-lab.html
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 21
> > Date: Wed, 2 Aug 2006 11:08:00 -0500
> > From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
> > Subject: [Histonet] charging and CPT questions
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <A2AD4F58AD06B44FBA71D4B1C4B9571A0680A395 <@t> mmcmail.ad.mhsil.com>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> >
> >
> > Can anyone shed any light on the proper way to code and charge the
> > following:
> >
> >
> >
> > 1.    Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319?
> > I'm not sure which one.
> >
> >
> >
> > 2.    Touch preps -  Done when we do a frozen section.   The frozen
> > section is 88331 and the touch prep should be 88333 or 88334?
> >
> >
> >
> >       3.   Semi-thin sectioning of nerve biopsies - Plastic embedding
> > In the past we charged all nerve bxs with an 88348 (EM) charge since
> >
> >                     not only did we do the semi-thin sectioning but 
> > we also did the transmission EM.  Our neuropathologist  now wants 
> > the semi-thin sections
> >
> >                      routinely but not the rest of the EM 
> > examination.
> >
> >
> >
> > Thanks
> >
> >
> >
> > Jim Vickroy
> >
> > Technical Supervisor - Surgical and Autopsy Pathology
> >
> > Memorial Medical Center
> >
> >
> >
> >
> >
> > This message (including any attachments) contains confidential 
> > information
>intended for a
> > specific individual and purpose, and is protected by law. If you are 
> > not
>the intended recipient,
> > you should delete this message. Any disclosure, copying, or 
> > distribution
>of this message, or the
> > taking of any action based on it, is strictly prohibited.
> >
> >
> > ------------------------------
> >
> > Message: 22
> > Date: Wed, 2 Aug 2006 09:45:21 -0700
> > From: "Jayne Scoggin" <jscoggin <@t> biocept.com>
> > Subject: [Histonet] please unsubscribe me from the list
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID: <200608021624.k72GO3Vh004624 <@t> mx.bioceptlabs.com>
> > Content-Type: text/plain;     charset="us-ascii"
> >
> > Please unsubscribe me from the list.
> >
> > Jayne Scoggin, Biocept Inc.
> > 858 320 8224
> > jscoggin <@t> biocept.com
> >
> >
> >
> >
> >
> > ------------------------------
> >
> > Message: 23
> > Date: Wed, 2 Aug 2006 12:40:55 -0400
> > From: "Zajic Kari" <Kari.Zajic <@t> HCAhealthcare.com>
> > Subject: RE: [Histonet] charging and CPT questions
> > To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
> >       <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >       <095327C7CDBDF64B9E9728A54799091E015CC5A2 <@t> ORLEV03.hca.corpad.net>
> > Content-Type: text/plain;     charset="iso-8859-1"
> >
> > Hi Jim. I do not do muscle or nerve at my facility but we send to 
> > the
>University of Miami and they charge the following:
> >
> > Muscle
> > 88305 x1: Surgical Path,gross,micro
> > 88313 x2: Paraffin: H&E Plastic
> > 88314 x2: cryostat H&E Trichrome
> > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, 
> > SDH
> >
> > Nerve
> > 88305 x1: Surg Path,gross,micro
> > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, 
> > Crystal
>Violet,Silver Stain
> > 88314 x2: Cryostat: H&E,Trichrome
> > 88319 x1: Frozen Section Histochem: Esterase
> > 88348 x1: Nerve Electron Micro
> > 88356 x1: Nerve Morphometry
> > 88362 x1: Nerve Teased Preps
> >
> > As far as frozen section touch preps, they came out with new CPT's 
> > in 2006
>as follows:
> > 88333 Path consult during surgery (cyto exam) initial site ie.touch
>prep,squash prep
> >       physician fee global $75-100+
> >       technical $17-25
> > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep
> >       physicain fee $40-50+
> >       technical $10-15
> > this info was given to me by Health Systems Concepts,Inc.
> >
> > hope this helps!!!
> > Kari :)
> >
> > Kari Marie Zajic HT,MLT
> > Histology Supervisor
> > Palms West Hospital
> > Pathology Department
> > 13001 State Road Eighty
> > Loxahatchee, Florida 33470
> > phone:      (561)798-6036
> > telefax:     (561)753-4298
> > voicemail: (561)753-4299
> > pager:       (561)610-4949
> > email: Kari.Zajic <@t> HCAHealthcare.com
> >
> > This email and any files transmitted with it may contain PRIVILEGED 
> > or
>CONFIDENTIAL
> > information and may be read or used only by the intended recipient. 
> > If you
>are not the intended recipient of the email or any of its attachment, 
>please be advised that you have received this email in error and that 
>any use, dissemination, distribution, forwarding, printing, or copying 
>of this email or any attached files is strictly prohibited. If you have 
>received this email in error, please immediately purge it and all 
>attachments and notify the sender by reply email or contact the sender 
>at the number listed.
> >
> >
> >
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu
> > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of 
> > Vickroy, Jim
> > Sent: Wednesday, August 02, 2006 12:08 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] charging and CPT questions
> >
> >
> >
> >
> > Can anyone shed any light on the proper way to code and charge the
> > following:
> >
> >
> >
> > 1.    Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319?
> > I'm not sure which one.
> >
> >
> >
> > 2.    Touch preps -  Done when we do a frozen section.   The frozen
> > section is 88331 and the touch prep should be 88333 or 88334?
> >
> >
> >
> >       3.   Semi-thin sectioning of nerve biopsies - Plastic embedding
> > In the past we charged all nerve bxs with an 88348 (EM) charge since
> >
> >                     not only did we do the semi-thin sectioning but 
> > we also did the transmission EM.  Our neuropathologist  now wants 
> > the semi-thin sections
> >
> >                      routinely but not the rest of the EM 
> > examination.
> >
> >
> >
> > Thanks
> >
> >
> >
> > Jim Vickroy
> >
> > Technical Supervisor - Surgical and Autopsy Pathology
> >
> > Memorial Medical Center
> >
> >
> >
> >
> >
> > This message (including any attachments) contains confidential 
> > information
>intended for a
> > specific individual and purpose, and is protected by law. If you are 
> > not
>the intended recipient,
> > you should delete this message. Any disclosure, copying, or 
> > distribution
>of this message, or the
> > taking of any action based on it, is strictly prohibited. 
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> >
> >
> > ------------------------------
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu 
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > End of Histonet Digest, Vol 33, Issue 2
> > ***************************************
> >
>
>
>_______________________________________________
>Histonet mailing list
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>_______________________________________________
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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