[Histonet] CD3 antibody for FFPE rabbit tissue

[C.M. van der Loos]

   Dear Narinder,

   It's a wild guess and haven't tried it, but perhaps worthwhile to test
   (....and better than nothing):

   The  LabVision  CD3 rabbit monoclonal antibody (clone SP7) reacts very
   well with  a  lot  of  different species (human, rat, mouse), so to my
   opinion there  is  a  good  chance  it reacts with rabbit CD3 as well.
   Just test  it  on  some  lymphoid rabbit tissue and look through heavy
   background staining (we have a rabbit-on-rabbit situation here and you
   will observe  a lot of plasmacells and staining near vessels!) whether
   or  not  T-cells are  positive. If this is the case you need the Zenon
   (Molecular    Probes/Invitrogen) rabbit-on-rabbit   kit   for   proper
   visualization. Please let us know if this approach works for you.

   Good luck!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Thu, 27 Apr 2006 12:02:53 -0700
   From: NDhatt <@t> qltinc.com
   Subject: [Histonet] CD3 antibody for  FFPE rabbit tissue
   To: histonetlistsutsouthwesternedu <histonet <@t> lists.utsouthwestern.edu>
   Hi All,
   I am looking for a CD3 antibody to use on FFPE rabbit tissue.  Most
   antibodies I have looked into are tested in human.
   Thanks
   Narinder Dhatt
   Preclinical - Histology
   QLT Inc.
   Vancouver, BC
   Canada