Appendix for Re: [Histonet] GRAM Stain Control Slides

[Gayle Callis]

Appendix works very well, but a veterinary laboratory situation, getting 
appendix may be difficult.  For vet diagnostic work, we used a section of 
bowel or try small intestine, do not rinse it excessively before fixation 
in NBF.  Cow, dog, pig, sheep, any animal should work - I would go for the 
species that is easiest to section.

There are several ways to make controls from pure cultures too and you can 
have your microbiology people help with this.    I think Histonet archives 
has some of these  methods, then you guarantee Gram pos and negative being 
there, and do a large number of blocks at one time.

At 09:42 AM 4/26/2006, you wrote:
>Sara:
>   I used for years sections from cases of acute appendicities. They 
> contain a host of both Gram + and - bacteria.
>   I hope this will help you!
>   René J.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)