[Histonet] fixing rat tibia

[Arfa R Maqsood]

Hi,
   Could anyone advise me regarding the time required to fix a rat 
tibia. Can it be fixed whole or is it best to slice in half longitudinally. 
Also is 70% ethanol appropriate as a fixative for bones that have 
been labelled with Oxytetracycline. Another dilema I have is that I 
dont have access to a specialist tools, should I need to bisect the 
tibia in half. What tools could be used to cut the tibia in half 
longitudinally without cracking the bone. I am new to all this so any 
any help / suggestions will be appreciated. Thanks
                             Ash 
ARFA.R.MAQSOOD
ENDOCRINE SCIENCE RESEARCH GROUP
UNIVERSITY OF MANCHESTER
OXFORD ROAD
MANCHESTER
M13 9PT

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