[Histonet] Cd34 in trephine bone marrow

[Mildred Fail]

 We use formic We use CD34 purchased from Becton-Dickinson. 1:200, citrate buffer for 20 minutes in the steamer. We also use formic acid to decalcify bone marrows. 

Rena Fail

>>> "Gudrun Lang" <gu.lang <@t> gmx.at> 04/25/06 03:41PM >>>
Hi,
Can anyone share a protocol for CD34 on decalcified FFPE human bone marrow
biopsies?
We have a benchmark XT and decalcify with formic acid.
Are there any hints especially refering to CD34 in acid-decalcified tissue?

Thank you

Gudrun Lang


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