[Histonet] Bone decalcification

[Smith, Allen]

Heather's question is not at all unreasonable.  Some acids are pretty decent
fixatives.  While I learned histotechnique back in the Dark Ages, I was
taught to fix and decalcify simulataneously.  We used 85% saturated aqueous
picric acid, 10% formalin, and 5% formic acid.  The explosive nature of dry
picric acid has made this formula less popular than it used to be.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Charles.Embrey
Sent: Monday, April 24, 2006 9:24 AM
To: Heather.A.Harper <@t> pcola.med.navy.mil
Cc: Histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Bone decalcification

I don't know what school you went to but I would want my money back.
The last thing you want is to put unfixed, fragile cells into acid and
allow them to rot while the bone decals. What would be left to fix
later?
Chuck

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Heather.A.Harper <@t> pcola.med.navy.mil
Sent: Monday, April 24, 2006 5:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone decalcification

  I just wanted to know other histo techs opinions on bone
decalcification.
I learned in school that you decal the bone than you fix it. I have a
pathologist who claims she learned it the hard way, and that it is
better to
fix the bone than decal it. What did you learn on how to do this
procedure?
This pathologist claims that fixing prior to decaling keeps the cells
more
intact. Any opinion on your procedure or your technique is appreciated.

 

Heather A. Harper          

Naval Hospital 

Pensacola, FL

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