[Histonet] colocalization of fluorescence

[Sarka Lhotak]

Hello Netters,

Sorry if I misunderstood this thread but it is not making much sense.
You will not be colocalizing colours, as in mixing the colors. You will
see and image each color separately through a different filter. You
might then superimpose the images on top of each other but it is not
necessary. It is usual to publish separate colors side by side.
The colors you will see in the microscope depend on your emission
filters. If you have a long pass filter you will see both red and green
with 488 excitation. With band filters you should only see one color at
a time. 
Hope this helps,

Sarka Lhotak
McMaster University, Hamilton, Ontario, Canada