[Histonet] Re: DAB precipitate

[Carl Hobbs]

I routinely make up my DAB powder in water and aliquot and freeze in 1.5ml
plastic m'fuge tubes..I never have any problems with it. Please see here
http://www.immunoportal.com/ for my method. Look under IHC methods...."DAB".
As I routinely use between 10 and 24 slides per run( manual) I always
develope my racked slides in a 200ml working solution of DAB, to minimise
contact. Pics of the plastic staining dishes on the Immunoportal site, too.

carl