[Histonet] FW: Help!

[Dawson, Glen]

-----Original Message-----
From: Bobrowitz, Carol [mailto:carolb <@t> phys.mcw.edu]
Sent: Friday, April 21, 2006 11:50 AM
To: Dawson, Glen
Subject: Help!



Glen, 

I would like to send the question below to the histonet but am having problems. 
Could you please post it for me. 

Have a great vacation. 

Thanks, 

Carol 

 -----Original Message----- 
From:   Bobrowitz, Carol  
Sent:   Friday, April 21, 2006 11:44 AM 
To:     HISTONET (E-mail) 
Subject:        repeating AR heat treatment 

Hello, 

I have already stained FFPE rat kidney's using FITC with a 2 hour heat treatment in citrate buffer ph 6.0.  (the staining was great)

I am now going to restain the same rat kidney slides with an additional primary using DAB as the chromagen.  This primary also requires heat treatment.

Am I correct in my thinking that I should repeat the heat treatment again due to the fact that the epitope (binding) sites have already closed after accepting the application of the 1st primary?

Any help will be appreciated.  Thank you in advance. 

Carol Ann Bobrowitz 
Medical College of Wisconsin 
Department of Physiology 
414-456-8179 
cbobrowi <@t> mcw.edu