[Histonet] RE: Ki67 protocol--frozen sections

[C.M. van der Loos]

   Dear Janelle,

   For   staining   of   Ki67  we  like  to  fix human  cryostat  tissues
   sections with  Zamboni's  or buffered  formalin.  This  results into a
   sharper  nuclear  staining than acetone-fixation. Just any polymer/HRP
   visualization and DAB+ as chromogen will do.

   As for a Ki67 antibody working on feline tissues I would recommend you
   to test the SP6 clone (rabbit monoclonal) from LabVision or Vector. In
   our  hands  this  clone already worked for a lot of different species.
   Perhaps you can expand the list with feline...

   Good luck!

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Thu, 20 Apr 2006 22:21:29 -0400
   From: "Janelle Novak" <gatorjanelle <@t> yahoo.com>
   Subject: [Histonet] Ki67 protocol--frozen sections
   To: [1]histonet <@t> lists.utsouthwestern.edu

   Does anyone have any good recommendations for a Ki67 staining protocol
   for feline frozen tissue sections?
   Thank you,
   Janelle Novak

References

   1. mailto:histonet <@t> lists.utsouthwestern.edu