[Histonet] bone marrow Fe stains

[Joyce Cline]

I use only purchased iron controls from H.O.M.E. I use the same control
for the aspirate block, the core block and the smears.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Galiotto, Laura
Sent: Wednesday, April 19, 2006 3:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bone marrow Fe stains

I have a problem with bone marrow aspirate controls. The problem is that
we are limited to the amount of controls we can collect.  Even though
the tissue control is working if the aspirate control (which are
difficult to obtain) does not work we are being asked to repeat the
stain until we find a aspirate control that is acceptable. Since I can
not guarantee the aspirate control contains an adequate amount of Fe, I
will be wasting time and reagents. 
Can anyone give feedback on how they process stains on bone marrow's, do
you only use a tissue control, does anyone know where bone marrow
aspirate controls -positive for Iron -can be purchased commercially? 

Laura Galiotto, HT (ASCP)
Histology Facilitator

Northwest Community Hospital 
800 West Central Road
Arlington Heights, Il 60005
847-618-6190

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