[Histonet] Expiry Period - Immunofluorescence Dilutions (Gagnon, Eric)

[Emma JONES]

Hi Eric,=20
Have you considered asking your Ventana rep for information on their =
FITC reagents. The expiry dates on those are far longer than any you =
will get from making your own up. Approximately 12 months +
regards

Emma Jones

Today's Topics:

   1. CD-3 woes (Favara, Cynthia (NIH/NIAID) [E])
   2. RE: mouse spleen confocal (Favara, Cynthia (NIH/NIAID) [E])
   3. Expiry Period  - Immunofluorescence Dilutions (Gagnon, Eric)
   4. Re: mouse spleen confocal (Gayle Callis)
   5. RE: Expiry Period  - Immunofluorescence Dilutions
      (Favara, Cynthia (NIH/NIAID) [E])
   6. RE: Expiry Period  - Immunofluorescence Dilutions (Dawson, Glen)
   7. Re: lendrums' phloxine/tartrazine (John Kiernan)
   8. Re: mouse spleen confocal (Scott Parker)
   9. RE: RE: RHS-1 and MicroMed T/T Mega/Jeanine (Joyce Cline)
  10. Re- Unscri be (Quadeer, Shahnaz S.)
  11. IEC CTD harris cryostat (dfs dsaf)
  12. Over lapped flouresence in double staining (sohail ejaz)
  13. RE: Cryostat (Molinari, Betsy)
  14. beginner questions on methacrylate resins (Nancy W. Troiano)
  15. M. leprae control slides (Bartlett, Jeanine)
  16. Microwave transparent plastics: Coleman Mfg contact
      information (Cheryl)
  17. Bouin's alternative/substitution question (jason.burrill <@t> crl.com)
  18. Re: Mouse lungs OCT and curling (Gayle Callis)


----------------------------------------------------------------------


=20



Message: 3
Date: Tue, 18 Apr 2006 13:23:28 -0400
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] Expiry Period  - Immunofluorescence Dilutions
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F93BD6329FC3AE4C8DB116B985FBC31309720673 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain;	charset=3D"iso-8859-1"

=20
=20
Hello Listers,
=20
We currently do immunofluorescence for IgG, IgA, IgM, C3, C1q, Fib., =
Kappa, Lambda, and C4d on renal biopsies, and the first four on skin =
biopsies.  We are using Dako Antibody Diluting Buffer and mostly Dako =
FITC antisera.
=20
We currently make up about 1 ml of each, and the dilutions last us =
between 1 and 3 weeks.  We would like to keep them longer if possible, =
making them up in larger quantities, possibly with the goal of =
transferring them to dispensers on our Ventana XT's.
]
Is there anyone doing the same immunofluorescence panel and dilutions, =
and if so, how long are you able to use your dilutions once made?
=20
Thanks for your assistance,
=20
Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada





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