[Histonet] lendrums' phloxine/tartrazine

[John Kiernan]

Lendrum's original paper explains the method very
thoroughly and has exact instructions for making
adjustments to the colour scheme. The reference is
LENDRUM, A. C. 1947. The phloxin-tartrazine method
as a general histological stain and for the
demonstration of inclusion bodies. Journal of
Pathology and Bacteriology 59, 399-404.
I strongly recommend reading this. Regarding
stability, the phloxin B solution can be kept for
one year; the saturated solution of tartrazine in
ethylene glycol monoethyl ether is stable for
years. It collects phloxin B with repeated re-use.
A more recent paper in this field is
CLARK, G. 1979. Displacement. Stain Technology 54:
111-119.
Clark investigated the method and came up with an
improved version using two different dyes. I've
seen only one published reference to the Clark's
method; perhaps most users are happy with
Lendrum's.

John Kiernan
Anatomy, UWO
London, Canada
____________________________
"Till, Renee" wrote:
> 
> Does anyone have experience with this stain? I have been trying it for a
> few weeks now on mouse jejunum. Tissues from some of my animals have
> wonderfully stained Paneth cells, while on others the Paneth cells are
> the first to be de-stained by the tartrazine. And these are all mouse
> jejunum from mice of the same age. Some do have a gene knockout, but I
> have not noticed the problem being only in the knockouts or only in the
> wild type, I am having problems with some from both. Any ideas? I have
> tried adjusting the time of the phloxine solution, but so far this
> hasn't helped any. Neither has making fresh (how long are these stains
> good for anyway?). Maybe there was more autolysis before fixation? I
> don't know. The times with the tartrazine solution have varied great on
> all the ones that have stained well. Anywhere from 5 minutes to over an
> hour.
> 
> 
> 
> 
> 
> Renee' Till, HT
> 
> 
> 
> Arkansas Childrens Nutrition Center
> 
> 1120 Marshall
> 
> Little Rock, AR
> 
> 72202
> 
> 
> 
> 
> 
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