[Histonet] IHC for NK cells in Balb/c mice?

[Koelling, Ray]

Robert,
you are correct NK1.1 works in BL/6 backround (and a few others like NZB)
but NOT in Balb/c, DBA, etc.  Clone 2B4 is nice but similar MHC restriction
to NK1.1 and is a mouse so you'd have mouse-on-mouse issues.  Might try
clone DX-5 which is a rat IgM and which is not restricted by haplotype and
works in Balb/c mice.  But have used these in flow and not in IHC so if you
try, I'd stick with frozen or something like Zinc/tris fixative.  And you
probably know, there is no known NK cell only marker known so far.  So in
IHC you'd be picking up subpopulations of other cells (like small set of
T's).  By flow we can gate out through dual and triple lables to see only
NK's.  
Ray

Raymond Koelling
Pathology Research Scientist
Amgen Corp.
Seattle, WA
  
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Fisher,
Robert
Sent: Friday, April 14, 2006 6:06 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] IHC for NK cells in Balb/c mice?


Can anyone suggest a protocol for staining NK cells in formalin-fixed,
paraffin-embedded Balb/c mouse tissue?  It is my understanding that the
NK1.1 antibody only works in BL/6 background mice (but I'd love to be
corrected on this point!).

Thanks,
Robert

Robert W. Fisher, Ph.D.
Staff Fellow
FDA/CBER/OBRR/DH/LPD
301-496-2579
Robert.Fisher <@t> fda.hhs.gov