[Histonet] AW: immunofluorescence

[Gudrun Lang]

Joe,
How long is it possible to store the slides in the refrigerator before
staining? One, two days?
Do you store them in simple boxes, or with a desiccant?

Gudrun Lang
Linz, Austria


-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Joe Nocito
Gesendet: Sonntag, 14. Mai 2006 02:42
An: Jodiputnam <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Betreff: Re: [Histonet] (no subject)

Jodi,
we cut ours on plus slides and let them air dry. We don't dip them in
acetone. If they are to be stained the next day, we leave them in a
refrigerator overnight. Hope this helps.

Joe Nocito BS, HT(ASCP)QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message -----
From: <Jodiputnam <@t> aol.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, April 12, 2006 4:29 PM
Subject: [Histonet] (no subject)


> Hi everyone. I have a few questions for you immunofluorescence experts out
> there. I recently have started doing IF on derm cases. I have noticed that
> quite  a few of my sections are washing off or folding. So far the doctor 
> has been
> able  to make a diagnosis on all of the them, but I would like to improve 
> my
> methods.  After I cut the frozens, I dip the slides in acetone and allow 
> them
> to dry  completely. I've noticed that the sections start coming off or 
> folding
> during my  PBS washes. I'm very gentle while rinsing but still have 
> problems.
> Any tips  would be very much appreciated. Its so frustrating to have
> beautiful sections at  the cryostat and end up with very little to show 
> for the
> finished product.
>
> Thanks!!
> Jodi
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