[Histonet] (no subject)
Cheryl
tkngflght <@t> yahoo.com
Wed Apr 12 16:59:26 CDT 2006
Hi Jodi-
As most of my methods are proven by practice, not by science, please try this on an old block first:
When you cut the section before dipping in a fixative, hold the slide's empty side against the back of your hand or arm (covered of course by a glove) to warm the OCT and 'melt' the section onto the charged slide. As soon as you see the OCT liquefy, drop it gently into the acetone. We've used 95% etOH as a fixative as well--seems to be a bit gentler on things that roll off...but again I caution--practice on a case that has been signed out as a parallel.
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one Tech at a time.
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