[Histonet] What is your favorite frozen section fixative

[Monfils, Paul]

For most immuno work, acetone or methanol. For strictly morphologic work,
10% formalin in 70% ethanol.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Stephen Peters M.D.
> Sent: 	Monday, April 10, 2006 7:31 AM
> To: 	Histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] What is your favorite frozen section fixative
> 
> HI histonetters,
>    
>   I would like to do a comparison of the morphologic results using
> different fixatives
>    for frozen section slides. I have always been happy with 95% ETOH. I
> know there are other good fixatives people use and I thought it would be
> useful to compare them. I will photograph and add these results to my 
>   web site tutorial (
> http://pathologyinnovations.com/frozen_section_technique.htm ) and frozen
> section lecture ( NSH Region 1 April 28 & 29 and California Society 
>   of Histotechnology May 18-21).  
>   Please let me know what you find is the best fixative for frozen section
> morphology.
>    
>   Stephen
>    
>    
>    
> 
> 
> Stephen Peters M.D. 
> Vice Chairman of Pathology
> Hackensack University Medical Center 
> 201 996 4836
>  
> Pathology Innovations, LLC 
> 410 Old Mill Lane, 
> Wyckoff, NJ 07481 
> 201 847 7600 
> www.pathologyinnovations.com 
> 
> 
> 
> 
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