[Histonet] toluidine blue staining

[Stefanie Werner]

Hello,

i have a problem with my toluidine blue staining after radioactive in situ 
hybridization. After developing the slides i stain them with 0,2% 
toluidine blue. After staining I often see under the microscope a red 
background colour on the slide and in the tissue. Therefore I can't see my 
radioactive signal properly. Did anybody have the same problem and can 
help me? I don't know what to do, because it just appears sometimes, even 
when the procedure is the same.

Thanks.

Steffi 

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