AW: [Histonet] suggestion for sections washing off

[Gudrun Lang]

Josie,
When we loose a section in the Ventana Benchmark it's mostly due to water
under the tissue. Often the sections dry from the margins and in the middle
a bit of water is trapped. The water prevents the tissue to attach the
glass. Therefore I try shake off the water before.
We put fatty slides in the 60 degree oven for 5 to 10 min before the run. 
I don't think that using an extra adhesive is a good idea, because the
machine is adjusted to the superfrost slides. Perhaps it would lead to an
uneven staining.

Gudrun Lang
Linz, Austria

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Nava,
Josefa
Gesendet: Donnerstag, 06. April 2006 14:22
An: histonet <@t> pathology.swmed.edu
Betreff: [Histonet] suggestion for sections washing off

Hello to Everyone,
 
I am running my IHC using  Ventana  stainer  . Sometimes  a positive charged
slide is not enough to hold the sections. Any suggestions that I can do to
the positive  charged slides?
Will  putting thin coat of Albumin to the positive charged slides do the
trick? I heard that it  will affect the positivity of the slide. Will it not
affect the immuno staining?
Any suggestion is very much appreciated.
 
 
Thanks,
Josie Nava


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