[Histonet] Mayer's hematox

[John A. Kiernan]

You should have said!  Its:

Haematoxylin  1G
Sodium iodate  0.2G
Potassium alum  50G
Citric acid  1G
Chloral hydrate  50G
Dist. water  1000ml

Allow the first 3 items to dissolve in the water
overnight. Add citric acid and chloral hydrate,
then boil for 5 mins. Ready for use when cool.

D & W call the chloral hydrate a "preservative,"
which is a statement I don't understand.
Preserving what? This matter has been discussed on
Histonet in the past (not by me), and the
assertion has been made that Mayer's haemalum
works just as well without the chloral hydrate. I
can't remember who said that; I've used only the
complete mixture myself. It would be worth
searching in www.histosearch.com 
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Julien Lambrey de Souza wrote:
> 
> Thanks,
> but actually I meant I needed the recipe for making Mayer's hematox
> according to Drury and Wallington (We don't have the book here and
> although I ordered it, I won't have it in time for the due dates...).
> 
> Julien.
> 
> Le 06-04-05, à 13:09, John A. Kiernan a écrit :
> 
> > From the 4th edition (1967), p.127:
> >
> > "Stain for 5-10 minutes."
> > --
> > -------------------------------
> > John A. Kiernan
> > Department of Anatomy and Cell Biology
> > The University of Western Ontario
> > London,   Canada   N6A 5C1
> >    kiernan[AT]uwo.ca
> >    http://publish.uwo.ca/~jkiernan/
> >    http://instruct.uwo.ca/anatomy/530/index.htm
> > _______________________________
> > Julien Lambrey de Souza wrote:
> >>
> >> Hello,
> >>
> >> Does anyone have the protocol for Mayer's hematox from Drury and
> >> Wallington's book (Carleton's histological techniques)? I need to
> >> compare with other protocols I have.
> >>
> >> Thanks'
> >>
> >> Julien Lambrey de Souza
> >> Research assistant, Evolutionary biology
> >> University of Quebec at Rimouski
> >>
> >> Tel: (418) 723-1986 #1714
> >> Fax: (418) 724-1849
> >>
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> >