[Histonet] MOHS
Heather.A.Harper <@t> pcola.med.navy.mil
Heather.A.Harper <@t> pcola.med.navy.mil
Thu Sep 29 12:24:54 CDT 2005
Message 24,
I know most MOHS start at $25 minimal no experience. Those who have
experience start at $30. Hope that helps.
Heather A. Harper
Supervisor of Histology
Naval Hospital of Pensacola, FL
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
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Subject: Histonet Digest, Vol 22, Issue 38
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Today's Topics:
1. Soft tissue digestion (Langiano, Lori, MS, HT)
2. Mayer's Haemalum reactive (Massimo)
3. RE: cpt codes for direct smears (Maray Weirauch)
4. Enzyme method for Re: [Histonet] Soft tissue digestion
(Gayle Callis)
5. RE: Supervisor-Anatomic Pathology (Orr, Rebecca)
6. for anyone in pharmaceutical, contract labs, etc (Linke_Noelle)
7. Re: Pap Pen Blues (Johnson, Teri)
8. Re: Pap Pen Blues (Andrea T. Hooper)
9. CPT FOR TOUCH PREPS (Riesen, Rebecca)
10. Re: Methyl Green (Johnson, Teri)
11. RE: cpt codes for direct smears (Baez, Janet)
12. antibody for collagen (Galina Deyneko)
13. TS 106 (sandosis <@t> uia.net)
14. querry (Arvind Pundir)
15. Fwd: [Histonet] querry (Ian Montgomery)
16. re: phosphate buffered saline or Tris PBS ?
(PW Howorth, Physiology)
17. "empty" cryosections! (Joanne Whitehead)
18. gold chloride (Jerry Duncan)
19. Re: gold chloride (Rene J Buesa)
20. RE: "empty" cryosections! (Favara, Cynthia (NIH/NIAID))
21. Fwd: [Histonet] gold chloride (Ian Montgomery)
22. Re: querry (Gayle Callis)
23. Re: re: phosphate buffered saline or Tris PBS ? (Gayle Callis)
24. Moh's Histotech salary range (Snyder, Wendy)
25. RE: querry (Monfils, Paul)
26. FW: [Histonet] "empty" cryosections! (Jasper, Thomas G.)
27. Re: Moh's Histotech salary range (Jackie M O'Connor)
----------------------------------------------------------------------
Message: 1
Date: Wed, 28 Sep 2005 10:04:52 -0700
From: "Langiano, Lori, MS, HT" <lori.langiano <@t> medtronic.com>
Subject: [Histonet] Soft tissue digestion
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <32D2979CA2119041B2A84241FCD860FD1C4E43 <@t> STSM1BMSGM01>
Content-Type: text/plain; charset="us-ascii"
Has anyone had experience with digesting soft tissue away from an
implant? I need to completely remove the tissue from an implant. Thanks
in advance for any suggestions.
Lori
------------------------------
Message: 2
Date: Wed, 28 Sep 2005 19:12:44 +0200
From: "Massimo" <andromeda_tm <@t> libero.it>
Subject: [Histonet] Mayer's Haemalum reactive
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<USER_ANONYMOUS$151.25.181.31$.000a01c5c44f$d8a2b710$1fb51997 <@t> SN300208440005
>
Content-Type: text/plain; charset="iso-8859-1"
Dear Histonetters,
I'd like to apologize if my question will sound too basic.
I have stained a tissue with Mayer's Haemalum reactive.
After I have put the specimen in water at a Ph a bit over 7, I would be
expected a nuclear colour change from red "bordeaux" to blue.
But it didn't happen.
I fixed the specimen with Bouin's liquid and I clarified with Histoclear.
I wonder why.
Have you seen a behaviour of this kind?
Thanks in advance for your help,
Best Regards,
Massimo
------------------------------
Message: 3
Date: Wed, 28 Sep 2005 13:18:02 -0400
From: "Maray Weirauch" <mweirauch <@t> crittenton.com>
Subject: RE: [Histonet] cpt codes for direct smears
To: <bwhitaker <@t> brownpathology.com>,
<histonet <@t> lists.utsouthwestern.edu>,
<Barb.Richmond <@t> mckennan.org>,<jnocito <@t> pathreflab.com>
Message-ID: <s33a9815.005 <@t> mail.crittenton.com>
Content-Type: text/plain; charset=US-ASCII
We have also been denied payment when using 88160 or 88161. The Centers
for Medicare and Medicaid Services (CMS) have taken the stand that
cytopathology codes 88160, 88161 and 88162 can not be reported for the
same patient on the same date of service with codes 88304 to 88309,
'Level III through VI - Surgical pathology, gross and microscopic
examination.' CMS said it considers cytopathology smears prepared from
surgical pathology specimens as duplicate procedures that should not be
reported separately. They will allow the use of modifier -59 for bypass
if the cytopathology smears are performed on separate specimens and
independent diagnoses are rendered.
That scenario never seems to apply to our situation, so we also bill
for a Pathology consultation during surgery, 88329.
>>> "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com> 9/27/2005 10:22:49
AM >>>
According to our billing company, we were being denied 88160 or 88161
if
permanent sections were being done on the same specimen. We are
billing an
88329 for those. What has everyone else's experience been?
Bonnie Whitaker
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Tuesday, September 27, 2005 9:05 AM
To: 'Barb Richmond'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] cpt codes for direct smears
We use 88160, other than gynecological for direct smears
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barb
Richmond
Sent: Monday, September 26, 2005 3:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cpt codes for direct smears
I need to know what cpt code to use for a direct smear that is made
from
tissue and stained on the quick frozen section stain line. Any
suggestions??
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Wed, 28 Sep 2005 11:22:12 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Enzyme method for Re: [Histonet] Soft tissue digestion
To: "Langiano, Lori, MS, HT" <lori.langiano <@t> medtronic.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050928111511.01b49278 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I used to digest soft tissue off skulls, long bones for museum teaching
pieces using an enzyme detergent. Make up 10 to 20% BIZ detergent in
water, heat to 80C in waterbath, and put bones, whatever in this for
several hours. It will even remove fixed tissue (if you rinse it well with
water before hand as NBF could inactivate the enzyme a bit. Do NOT boil.
Rinse well with water afterwards, and soft tissue will release from bone,
whatever. Beware, it will eat cartilage off bones. You can repeat until
the implant is clean. Try to manipulate soft tissues off with gloved
hands, or soft brushes. Best part of all this, is isn't smelly, and bones
come out perfectly clean and white.
I have a reference for this from J Microscopy, many moons ago.
At 11:04 AM 9/28/2005, you wrote:
>Has anyone had experience with digesting soft tissue away from an
>implant? I need to completely remove the tissue from an implant. Thanks
>in advance for any suggestions.
>Lori
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 5
Date: Wed, 28 Sep 2005 13:13:00 -0500
From: "Orr, Rebecca" <ROrr <@t> enh.org>
Subject: [Histonet] RE: Supervisor-Anatomic Pathology
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A7FC3A4F98964A44B0B71B7B25EA6F96055B6DC8 <@t> EXCHANGE03.enhnet.org>
Content-Type: text/plain; charset="us-ascii"
HI everyone,
Anyone interested?
It's a great place to work!
Becky Orr CLA,HT(ASCP)
IHC Lead
Evanston Northwestern Healthcare
847-570-2771
-----Original Message-----
From: Wagner, Nikole
Sent: Wednesday, September 28, 2005 8:29 AM
To: Orr, Rebecca
Subject: Supervisor-Anatomic Pathology
Evanston Northwestern Healthcare is seeking a Laboratory Supervisor for
our Anatomic Pathology Department at Evanston Hospital. The position
requires HTL (ASCP) certification and 3-5 years of supervisory
experience in a Histology Laboratory. Interested individuals can
submit resumes to nwagner <@t> enh.org or apply on-line at
www.enh.org/careers.
Nikole Wagner
Sr. Human Resources Associate
Evanston Northwestern Healthcare
Phone. 847-570-1484
Fax. 847-570-1903
------------------------------
Message: 6
Date: Wed, 28 Sep 2005 12:20:10 -0700
From: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Subject: [Histonet] for anyone in pharmaceutical, contract labs, etc
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D475C767BF2F6D438FD30E3984FCBB110ED14B <@t> irmail121.irvine.allergan.com>
Content-Type: text/plain; charset="us-ascii"
Hi All,
I have a question regarding necropsy. Do you typically have one organ
weigher, or does each individual do their own weighing? How many
computers do you have set up in your necropsy rooms?
Thank you!!
Noelle Linke, BS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568
------------------------------
Message: 7
Date: Wed, 28 Sep 2005 15:08:30 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: Pap Pen Blues
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E78016CB711 <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
For What It's Worth (FWIW), I pretty well despise Pap pens. And I
dearly LOVE Gayle's technical term (goo) for the stuff inside them.
They're expensive, but see if you can get various companies to send you
one to try. We have used them from Dako, Vector, and Biocare. We don't
use them any more. We have the coverplates from Shandon and that works
well for our hand-staining.
Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133
Andrea,
We now use Vectors ImmEdge pens - doesn't come up, and has a hint of
color. Cheaper, two to a set. Can remark a slide when wet, although we
do
blot away moisture, but the pen goo does not lift nor flow across a
section
if it contacts buffer - been there and have experienced the "Pap pen oil
slick syndrome" that ruins IHC when section gets goo-coated.
To use ImmEdge, be sure to shake pens very vigorously to redistribute
the
goo (per their instructions). We vortex them hard to do that job.
Maybe
the pens you have now need a good vortexing - worth a try for pricey
little
pens. Pressing the point to start goo is still something that requires
gentle tough, but I have not had too many problems with that. Flow is
generally excellent, right amount.
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
At 09:29 AM 9/28/2005, you wrote:
>Hello All,
>
>I have used Zymed Mini Pap pens for years now as through trials and
>tribulations and talking with coworkers we agreed it was the best out
>there. The pen rarely came up or leaked and gave beautiful results.
Even
>withstood heat retrieval in DAKO Target Retrieval Solution with no
>problems. The stuff was indestructible!!
>
>This is until recently ... I have noticed with the past several orders
>that the formula has changed. The pap pen "ink" is now a blue green in
>color (used to be more generic yellow/beige) and it does not stay on
well
>at all. I end up going home in a state of frustration every day as I
pap
>pen hundreds of slides with little success for the entirety of my
>experiment. Arggggh!
>
>So I have two questions:
>
>(1) Has anyone else noticed this change in formula over the past year
>or
>so? (It may have been longer as I was working with a stock of pap pens
>before that)
>(2) Who makes your favorite pap pen?
>
>THANKS!
>Andrea
>--
>
>________________________________
------------------------------
Message: 8
Date: Wed, 28 Sep 2005 16:27:25 -0400
From: "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu>
Subject: [Histonet] Re: Pap Pen Blues
To: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID: <p06200709bf60ac88a80d@[140.251.146.71]>
Content-Type: text/plain; charset=us-ascii; format=flowed
Hi Teri,
Thanks for the reply. Coverplates are great except for when, like in
my case, you have 3-6 sections per slide each to be stained with a
different antibody or dilution thereof!
Thanks for the info,
Andrea
>For What It's Worth (FWIW), I pretty well despise Pap pens. And I
>dearly LOVE Gayle's technical term (goo) for the stuff inside them.
>
>They're expensive, but see if you can get various companies to send you
>one to try. We have used them from Dako, Vector, and Biocare. We don't
>use them any more. We have the coverplates from Shandon and that works
>well for our hand-staining.
>
>Teri Johnson
>Managing Director Histology Facility
>Stowers Institute for Medical Research
>1000 E. 50th St.
>Kansas City, MO 64133
--
------------------------------
Message: 9
Date: Wed, 28 Sep 2005 17:06:59 -0400
From: "Riesen, Rebecca" <rebecca.riesen <@t> dsilabs.com>
Subject: [Histonet] CPT FOR TOUCH PREPS
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<05844092236E7749A1BBBCB1077C34A2DEA0AF <@t> dsi-ex01.gateway.dom>
Content-Type: text/plain; charset="us-ascii"
I just attended a meeting on coding and compliance at NSH in Ft. Lauderdale
and this was one of the topics. It was suggested that the code 88161 be
used for touch preps, with the warning that if you use it along with a code
for a frozen section it will be thrown out. You can only code for the touch
prep when there was no actual frozen section performed. My understanding
was that you CAN code for permanents and touch preps together, just not the
frozens and the touch preps. I hope this was helpful and if I misunderstood
at the meeting, please feel free to correct me. THANKS!
DSI Labs Email Disclaimer:
Confidentiality Notice: This e-mail message, including any attachments, is
for the sole use of the intended recipients(s) and may contain confidential
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distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of the original
message.
------------------------------
Message: 10
Date: Wed, 28 Sep 2005 16:11:12 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] Re: Methyl Green
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C28BAF593DC3314E9C0F3A50191C2E78016CB712 <@t> EXCHKC03.stowers-institute.org>
Content-Type: text/plain; charset="us-ascii"
Chris makes some very good suggestions. We have found that tissues
subjected to HIER don't want to take up the green stain using the usual
staining procedures, but had not found the "magic bullet" to get them to
stain satisfactorally. Other samples (no pretreatment or enzyme
pretreated) counterstain very well with methyl green.
Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133
------------------------------
Message: 11
Date: Wed, 28 Sep 2005 14:13:58 -0700
From: "Baez, Janet" <jbaez <@t> interscopepath.com>
Subject: RE: [Histonet] cpt codes for direct smears
To: "Maray Weirauch" <mweirauch <@t> crittenton.com>,
<bwhitaker <@t> brownpathology.com>, <histonet <@t> lists.utsouthwestern.edu>,
<Barb.Richmond <@t> mckennan.org>, <jnocito <@t> pathreflab.com>
Message-ID:
<9E956D8FEB06C2408B08AC16498325E9FE43 <@t> adsl-67-113-77-28.dsl.lsan03.pacbell.n
et>
Content-Type: text/plain; charset="us-ascii"
We also bill for an 88329.
Janet E. Baez
Histology Manager
Interscope Pathology
Canoga Park, Ca.
-----Original Message-----
From: Maray Weirauch [mailto:mweirauch <@t> crittenton.com]
Sent: Wednesday, September 28, 2005 10:18 AM
To: bwhitaker <@t> brownpathology.com; histonet <@t> lists.utsouthwestern.edu;
Barb.Richmond <@t> mckennan.org; jnocito <@t> pathreflab.com
Subject: RE: [Histonet] cpt codes for direct smears
We have also been denied payment when using 88160 or 88161. The Centers
for Medicare and Medicaid Services (CMS) have taken the stand that
cytopathology codes 88160, 88161 and 88162 can not be reported for the
same patient on the same date of service with codes 88304 to 88309,
'Level III through VI - Surgical pathology, gross and microscopic
examination.' CMS said it considers cytopathology smears prepared from
surgical pathology specimens as duplicate procedures that should not be
reported separately. They will allow the use of modifier -59 for bypass
if the cytopathology smears are performed on separate specimens and
independent diagnoses are rendered.
That scenario never seems to apply to our situation, so we also bill
for a Pathology consultation during surgery, 88329.
>>> "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com> 9/27/2005 10:22:49
AM >>>
According to our billing company, we were being denied 88160 or 88161
if
permanent sections were being done on the same specimen. We are
billing an
88329 for those. What has everyone else's experience been?
Bonnie Whitaker
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joe
Nocito
Sent: Tuesday, September 27, 2005 9:05 AM
To: 'Barb Richmond'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] cpt codes for direct smears
We use 88160, other than gynecological for direct smears
Joe Nocito, BS, HT(ASCP) QIHC
Histology Manager
Pathology Reference Lab
San Antonio, TX
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Barb
Richmond
Sent: Monday, September 26, 2005 3:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cpt codes for direct smears
I need to know what cpt code to use for a direct smear that is made
from
tissue and stained on the quick frozen section stain line. Any
suggestions??
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 28 Sep 2005 15:00:08 -0700 (PDT)
From: Galina Deyneko <galinadeyneko <@t> yahoo.com>
Subject: [Histonet] antibody for collagen
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050928220008.47992.qmail <@t> web33106.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear colleagues,
Would you please share information about antibodies for detecting collagen (
I do not specify type) and pre-collagen in mouse's heart and aorta FFPE
tissues. I looked through the archive, but I did not find an answer. We need
some additional sensitive test for collagen besides Masson Trichrome and
Sirius Red stainings.
Any suggestions, protocol, vendors would be highly appreciated.
Thank you at advance .
Galina Deyneko
Novartis Cambridge MA
617-871-7613.
---------------------------------
Yahoo! for Good
Click here to donate to the Hurricane Katrina relief effort.
------------------------------
Message: 13
Date: Wed, 28 Sep 2005 18:00:00 EST
From: <sandosis <@t> uia.net>
Subject: [Histonet] TS 106
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <200509282300.j8SN02T50259 <@t> smtp2.uia.net>
Hello,
Is there anyone out there who is doing the TS 106 (Thymidylate
Synthase)using the Ventana Benchmark?
I've found the antibody at Zymed (IVD) and Chemicon (RUO)
Would appreciate any info on:
Source: (Zymed vs. Chemicon vs. others)
Dilution:
Antigen Retrieval: (CC1, CC2, mild, standard, extended)
Antibody time:
Thanks so much for sharing the results of your hard work.
Sandra
---------------------------------------------
This message was sent using the UIA Web Mail Server.
ULTIMATE Internet Access, Inc http://www.uia.net/
------------------------------
Message: 14
Date: Thu, 29 Sep 2005 10:09:04 +0530
From: "Arvind Pundir" <arvind <@t> nbrc.res.in>
Subject: [Histonet] querry
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A67B43DEB48F364EA89D90599E0844E405CC64 <@t> mail>
Content-Type: text/plain; charset="utf-8"
can any one pleas tell protocol for subbing slides with gelatin with some
extra attachment property actually my tissue are comming off from the slides
thanks
Arvind Singh Pundir
National Brain Rsearch Centre
gurgaon, INDIA
------------------------------
Message: 15
Date: Thu, 29 Sep 2005 08:53:57 +0100
From: Ian Montgomery <ian.montgomery <@t> bio.gla.ac.uk>
Subject: Fwd: [Histonet] querry
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.2.3.4.2.20050929084427.030e3a30 <@t> udcf.gla.ac.uk>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Arvind,
Gelatine. = 1g.
Chrome Alum. = 0.1g. (Chromic potassium sulphate)
Distilled water. = 200ml.
Dissolve the gelatine in the water using gentle heat then
add the chrome alum. Coat the slides by dipping into the solution,
draining off excess then drying in an oven. For large numbers of
slides I keep clean staining racks specifically for subbing.
Ian.
>can any one pleas tell protocol for subbing slides with gelatin with
>some extra attachment property actually my tissue are comming off
>from the slides
>
>
>
>thanks
>
>
>
>Arvind Singh Pundir
>
>National Brain Rsearch Centre
>
>gurgaon, INDIA
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery <@t> bio.gla.ac.uk
------------------------------
Message: 16
Date: Thu, 29 Sep 2005 10:54:23 +0100
From: "PW Howorth, Physiology" <patrick.howorth <@t> bristol.ac.uk>
Subject: [Histonet] re: phosphate buffered saline or Tris PBS ?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <D0902CBE53A5209CDD28E459 <@t> pys-aep6.pys.bris.ac.uk>
Content-Type: text/plain; charset=us-ascii; format=flowed
Hi,
I'm currently using immunohistochemistry (free floating sections or
serially mounted sections) to map the rat brain. We use viral injections
to express green fluorescent protein and use 1 or 2 other labels (i.e. Cy3
or AMCA) to colocalise receptors with GFP expression.
I have been trying to improve our methods that we currently use.
Originally we used Tris PBS (0.01M, pH 7.4), I notice that other groups use
a variety of differences in these recipes, what advantages does Tris PBS
offer (if any) or would you recommend using PBS (either 0.1M or 0.01M) ?
Secondly, when using triton x-100 (0.3%) has anyone noticed a reduction
with GFP signal compared to just using 50% ethanol ?
And finally, we use cardiac perfusion to initially fix tissue followed by 2
hours post fixation with 4% phosphate buffered (0.1M, pH 7.4) formalin.
The antibody we use for mu opioid receptors (diasorin) - does anyone have
any experience with this antibody and this method have you done antigen
retrieval to improve the signal ?
Many thanks
Patrick
----------------------
Patrick Howorth,
Dept of Physiology,
University of Bristol,
Bristol,
BS8 1TD.
+44 (0)117 33 17112
patrick.howorth <@t> bristol.ac.uk
------------------------------
Message: 17
Date: Thu, 29 Sep 2005 07:38:22 -0500
From: Joanne Whitehead <joannew <@t> bluebottle.com>
Subject: [Histonet] "empty" cryosections!
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1127997502.433be03ea13c6 <@t> bluebottle.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi everyone,
I'm relatively new at cryosectioning, and so far have found it to be a
very frustrating experience! Some days it goes very smoothly and I get
plenty of nice sections, but most often I feel like it's a battle of
wills with the cryostat, which ususally wins.
My major problem is getting "empty" sections - the media cuts smoothly
but the tissue itself curls or crumples up, leaving just an open
circle of media. The other difficulty is with sections wrinkling as
they come off the knife, instead of lying flat. Does anyone know why
this happens, or how I can prevent it?
I'm sectioning mouse intestinal tissue, which has been rolled into a
"Swiss roll", fixed in paraformaldehyde, infused with sucrose, snap
frozen in isopentane over liquid nitrogen and embedded in OTC. I have
tried cutting at different temperatures, from -18C to -30C, with my
samples equilibrating in the machine at least an hour before I start.
I have seen protocols in which the tissue is embedded in OTC before
freezing, and also infusing the tissue with a mixture of sucrose and
OTC before embedding. Does anyone have any preference for these
methods?
I would really appreciate any advice!
Thank you!
Joanne
Curie Institute, Paris
------------------------------
Message: 18
Date: Thu, 29 Sep 2005 08:52:28 -0500
From: Jerry Duncan <jerryd <@t> dcla.com>
Subject: [Histonet] gold chloride
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6E1A8C242B4D6447A14A170D98A45C63EF18 <@t> barracuda.dcla.com>
Content-Type: text/plain; charset="iso-8859-1"
Fellow histologists,
We currently prepare gold chloride rather than purchase the prepared liquid.
Does gold chloride need to be refrigerated after preparation?
Thanks,
Jerry
------------------------------
Message: 19
Date: Thu, 29 Sep 2005 07:12:44 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] gold chloride
To: Jerry Duncan <jerryd <@t> dcla.com>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050929141244.92706.qmail <@t> web61218.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I not only did not refrigerate it, but I used it several times until the
pale yellow color began to fade. Never had any problems by doing that.
Rene J.
Jerry Duncan <jerryd <@t> dcla.com> wrote:
Fellow histologists,
We currently prepare gold chloride rather than purchase the prepared liquid.
Does gold chloride need to be refrigerated after preparation?
Thanks,
Jerry
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
---------------------------------
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Click here to donate to the Hurricane Katrina relief effort.
------------------------------
Message: 20
Date: Thu, 29 Sep 2005 10:47:07 -0400
From: "Favara, Cynthia (NIH/NIAID)" <cfavara <@t> niaid.nih.gov>
Subject: RE: [Histonet] "empty" cryosections!
To: 'Joanne Whitehead' <joannew <@t> bluebottle.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<A985B7D45D355D4EB363288E7173943709E02F <@t> NIHCESMLBX5.nih.gov>
Content-Type: text/plain
Welcome to the world of histology - Gayle Callis is the expert to guide you.
If she does not respond contact her directly!
Hang in there it does get better!
x
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives
-----Original Message-----
From: Joanne Whitehead [mailto:joannew <@t> bluebottle.com]
Sent: Thursday, September 29, 2005 5:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] "empty" cryosections!
Hi everyone,
I'm relatively new at cryosectioning, and so far have found it to be a
very frustrating experience! Some days it goes very smoothly and I get
plenty of nice sections, but most often I feel like it's a battle of
wills with the cryostat, which ususally wins.
My major problem is getting "empty" sections - the media cuts smoothly
but the tissue itself curls or crumples up, leaving just an open
circle of media. The other difficulty is with sections wrinkling as
they come off the knife, instead of lying flat. Does anyone know why
this happens, or how I can prevent it?
I'm sectioning mouse intestinal tissue, which has been rolled into a
"Swiss roll", fixed in paraformaldehyde, infused with sucrose, snap
frozen in isopentane over liquid nitrogen and embedded in OTC. I have
tried cutting at different temperatures, from -18C to -30C, with my
samples equilibrating in the machine at least an hour before I start.
I have seen protocols in which the tissue is embedded in OTC before
freezing, and also infusing the tissue with a mixture of sucrose and
OTC before embedding. Does anyone have any preference for these
methods?
I would really appreciate any advice!
Thank you!
Joanne
Curie Institute, Paris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Thu, 29 Sep 2005 15:59:41 +0100
From: Ian Montgomery <ian.montgomery <@t> bio.gla.ac.uk>
Subject: Fwd: [Histonet] gold chloride
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.2.3.4.2.20050929155750.030e4680 <@t> udcf.gla.ac.uk>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Jerry,
All I do is store in an amber bottle at ambient temperature.
Been doing it since I was a boy with no detriment.
Ian.
>Fellow histologists,
>
>We currently prepare gold chloride rather than purchase the prepared
liquid.
>Does gold chloride need to be refrigerated after preparation?
>
>Thanks,
>Jerry
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07623 975451
e-mail: ian.montgomery <@t> bio.gla.ac.uk
------------------------------
Message: 22
Date: Thu, 29 Sep 2005 09:10:55 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] querry
To: "Arvind Pundir" <arvind <@t> nbrc.res.in>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050929090120.01b541d0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I only use chrome gelatin for decalcified bone sections IF the plus charge
(Silane coated) slides DO NOT work. You can make it up with different
bloom gelatins (bloom indicating size of gelatin molecule 100 small, 275
and 300 large). The reason I do not care for gelatin, is background
staining with hematoxylin, can be reduced by dipping presubbed slides in
NBF 10 dips, rinsing well and storing in cool dry box.
DO NOT COAT SILANE aka PLUS CHARGE SLIDES, it is an extra expense and the
gelatin (protein) goes over the top of the Plus coating, rendering it
useless. This is clearly stated in package inserts with Plus Charge slides.
5gm gelatin in 1 liter distilled water, add 1 gm chromium potassium
sulfate, dip clean microscope glass slides in this,
air dry and store in a cool dry place. You can these slides like regular
slide to pick up section and do not add anything to waterbath, additional
adhesives will cause more unsightly background.
OR
Add 10 mls of this stock subbing solution to a 2 liter waterbath, like
adding commerically available adhesives
to a waterbath, dry sections in normal way - We dry bone sections FLAT at
37-40C for a couple of days, overnight is too minimal. Soft tissues can be
dried in an oven.
Chromium is considered very toxic, so use precautions when preparing the
subbing solution. We preferred the waterbath method when doing a large
number of blocks. Lessens preparation time to just have it in a waterbath
already. We only use this subbing solution with soft tissue as a backup
when all else fails to keep sections on a slide. We never use it for
immunstaining purposes.
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 23
Date: Thu, 29 Sep 2005 09:20:12 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: Re: [Histonet] re: phosphate buffered saline or Tris PBS ?
To: "PW Howorth, Physiology" <patrick.howorth <@t> bristol.ac.uk>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050929091209.01b667a8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
You can use either buffer for immunofluorescence work. We buy Dulbeccos
PBS from Sigma or make up TBS. There are other PBS recipes that will work
too. Our TBS is 0.05M concentratin, commonly used for IFA and IHC work.
TBS (TRIS BUFFERED SALINE)
STOCK 10X TBS
TRIS Base (FW 121.41) 0.5M 60.57 g
Sodium Chloride 89 - 90 g
Dissolve in 800 ml distilled water
Add 30 ml Hydrochloric Acid to adjust pH to 7.8, use magnetic stirrer with
pH meter.
Adjust final volume to 1 liter and store in refrigerator. If this becomes
cloudy, toss and make new. Good for 1 year.
0.05M TBS WORKING BUFFER
Dilute Stock 10X Buffer 1:10
100 ml Stock 10X TBS and 900 ml distilled water.
Final pH can be 7.6 - 7.8, final concentration of TBS is 0.05M in 0.9%
sodium chloride.
TBS is a preferred buffer when you work with alkaline phosphatase
immunohistochemical staining to eliminate phosphate ions that cause
background staining with substrate/chromogen, but with IFA work, no real
problems.
GFP can be rencered nonfluoresceing by certain things, solvents are one so
we never use detergents. You can find out more about GFP by going to
Clontech website and accessing Living Colours Manual, free and in pfd
format.
Personally and if you notice a reduction in GFP signal by using certain
reagents, I would eliminate them or reduce the concentration.
t 03:54 AM 9/29/2005, you wrote:
>Hi,
>
>I'm currently using immunohistochemistry (free floating sections or
>serially mounted sections) to map the rat brain. We use viral injections
>to express green fluorescent protein and use 1 or 2 other labels (i.e. Cy3
>or AMCA) to colocalise receptors with GFP expression.
>
>I have been trying to improve our methods that we currently use.
>Originally we used Tris PBS (0.01M, pH 7.4), I notice that other groups
>use a variety of differences in these recipes, what advantages does Tris
>PBS offer (if any) or would you recommend using PBS (either 0.1M or 0.01M)
?
>
>Secondly, when using triton x-100 (0.3%) has anyone noticed a reduction
>with GFP signal compared to just using 50% ethanol ?
>
>And finally, we use cardiac perfusion to initially fix tissue followed by
>2 hours post fixation with 4% phosphate buffered (0.1M, pH 7.4) formalin.
>The antibody we use for mu opioid receptors (diasorin) - does anyone have
>any experience with this antibody and this method have you done antigen
>retrieval to improve the signal ?
>
>Many thanks
>
>Patrick
>
>----------------------
>Patrick Howorth,
>Dept of Physiology,
>University of Bristol,
>Bristol,
>BS8 1TD.
>+44 (0)117 33 17112
>patrick.howorth <@t> bristol.ac.uk
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 24
Date: Thu, 29 Sep 2005 11:37:38 -0400
From: "Snyder, Wendy" <SnyderW <@t> uhcwv.org>
Subject: [Histonet] Moh's Histotech salary range
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CED048E760950D4FA48682F114EE91C3E553 <@t> uhc-exchange.uhc.wvuhs.com>
Content-Type: text/plain; charset="iso-8859-1"
My hospital is in the process of starting a Moh's Surgery program. We will
be hiring an experienced Moh's histotech, HT(ASCP) soon. I know the demand
for such a position is high. Could anyone give me an idea of the salary
range that a histotech gets paid for doing Moh's frozen sections? I would
really appreciate it.
Wendy Snyder HT(SCP)
United Hospital Center
Carksburg, WV 26301
------------------------------
Message: 25
Date: Thu, 29 Sep 2005 11:38:30 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] querry
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE017175E6 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
Making up your own chrome gelatin is a bit tedious, though I did so for
years before commercial products became available. Surgipath sells a chrome
gelatin product called STA-ON, which I have used for several years now. A
small amount of the solution added to the water bath works very well for
many kinds of hard tissue, as well as necrotic tissue, blood clots, and
other materials which tend to detach from the slide. It also works well on
sections of polymers and other foreign substances, which the average
clinical lab doesn't deal with very much, but which we have to section
frequently since we are a core research facility. "Plus slides" don't work
on such materials, since these materials usually do not possess the net
negative charge that most tissues have.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Gayle
> Callis
> Sent: Thursday, September 29, 2005 8:10 AM
> To: Arvind Pundir; Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] querry
>
> I only use chrome gelatin for decalcified bone sections IF the plus charge
>
> (Silane coated) slides DO NOT work. You can make it up with different
> bloom gelatins (bloom indicating size of gelatin molecule 100 small, 275
> and 300 large). The reason I do not care for gelatin, is background
> staining with hematoxylin, can be reduced by dipping presubbed slides in
> NBF 10 dips, rinsing well and storing in cool dry box.
>
> DO NOT COAT SILANE aka PLUS CHARGE SLIDES, it is an extra expense and the
>
> gelatin (protein) goes over the top of the Plus coating, rendering it
> useless. This is clearly stated in package inserts with Plus Charge
> slides.
>
> 5gm gelatin in 1 liter distilled water, add 1 gm chromium potassium
> sulfate, dip clean microscope glass slides in this,
> air dry and store in a cool dry place. You can these slides like regular
> slide to pick up section and do not add anything to waterbath, additional
> adhesives will cause more unsightly background.
>
> OR
>
> Add 10 mls of this stock subbing solution to a 2 liter waterbath, like
> adding commerically available adhesives
> to a waterbath, dry sections in normal way - We dry bone sections FLAT at
> 37-40C for a couple of days, overnight is too minimal. Soft tissues can
> be
> dried in an oven.
>
> Chromium is considered very toxic, so use precautions when preparing the
> subbing solution. We preferred the waterbath method when doing a large
> number of blocks. Lessens preparation time to just have it in a waterbath
>
> already. We only use this subbing solution with soft tissue as a backup
> when all else fails to keep sections on a slide. We never use it for
> immunstaining purposes.
> Gayle Callis
> Research Histopathology Supervisor
> Veterinary Molecular Biology
> Montana State University - Bozeman
> PO Box 173610
> Bozeman MT 59717-3610
> 406 994-6367
> 406 994-4303 (FAX)
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 26
Date: Thu, 29 Sep 2005 11:03:54 -0500
From: "Jasper, Thomas G." <TJasper <@t> smdc.org>
Subject: FW: [Histonet] "empty" cryosections!
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1DB04B57B04C5747B87C3B39F3E605AA07D3907B <@t> harrier>
Content-Type: text/plain; charset="iso-8859-1"
Thomas Jasper HT(ASCP)BAS
Anatomic Pathology Coordinator
SMDC Clinical Laboratory
Duluth, MN
tjasper <@t> smdc.org
-----Original Message-----
From: Jasper, Thomas G.
Sent: Thursday, September 29, 2005 11:03 AM
To: 'Favara, Cynthia (NIH/NIAID)'; ',histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] "empty" cryosections!
Dear Joanne,
One possible obstacle to optimal frozen tissue sectioning may be the
fixation step prior to freezing. I have not used paraformaldehyde to fix
and then freeze tissue for cryosectioning. However, I have been asked to
cut frozen sections on tissue that has been formalin fixed. The degree of
difficulty seems to increase the longer the tissue has been in a fixative.
In other words, if we get it out of formalin quickly (within minutes) we'll
probably get a decent section. If half the day has gone by we probably
wouldn't try. Once a section is obtained the main problem is usually one of
adherence.
This experience is in a clinical setting, with human tissue, which leads me
to my next point.
Generally speaking, animal tissue is much drier, and therefore more
difficult to section period. Also, if you are getting empty sections (no
tissue) you may be having a problem with your supporting media (OCT, etc.).
If the media is not adhering well to the tissue, it would lend itself to
creating these holes. Fatty tissue is difficult to freeze as well, although
I note that you have tried sectioning at different temperatures. I believe
I have seen postings from others to drop the temperatures very low to help
freeze the fatty tissues. I know there are others monitoring the Histonet
with more knowledge about frozen sectioning. I just thought I'd share some
of my experiences, hopefully it will be of some help. Good luck to you!
tj
Thomas Jasper HT(ASCP)BAS
Anatomic Pathology Coordinator
SMDC Clinical Laboratory
Duluth, MN
tjasper <@t> smdc.org
-----Original Message-----
From: Favara, Cynthia (NIH/NIAID) [mailto:cfavara <@t> niaid.nih.gov]
Sent: Thursday, September 29, 2005 9:47 AM
To: 'Joanne Whitehead'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] "empty" cryosections!
Welcome to the world of histology - Gayle Callis is the expert to guide you.
If she does not respond contact her directly!
Hang in there it does get better!
x
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317
Disclaimer:
The information in this e-mail and any of its attachments is confidential
and may contain sensitive information. It should not be used by anyone who
is not the original intended recipient. If you have received this e-mail in
error please inform the sender and delete it from your mailbox or any other
storage devices. National Institute of Allergy and Infectious Diseases shall
not accept liability for any statements made that are sender's own and not
expressly made on behalf of the NIAID by one of its representatives
-----Original Message-----
From: Joanne Whitehead [mailto:joannew <@t> bluebottle.com]
Sent: Thursday, September 29, 2005 5:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] "empty" cryosections!
Hi everyone,
I'm relatively new at cryosectioning, and so far have found it to be a
very frustrating experience! Some days it goes very smoothly and I get
plenty of nice sections, but most often I feel like it's a battle of
wills with the cryostat, which ususally wins.
My major problem is getting "empty" sections - the media cuts smoothly
but the tissue itself curls or crumples up, leaving just an open
circle of media. The other difficulty is with sections wrinkling as
they come off the knife, instead of lying flat. Does anyone know why
this happens, or how I can prevent it?
I'm sectioning mouse intestinal tissue, which has been rolled into a
"Swiss roll", fixed in paraformaldehyde, infused with sucrose, snap
frozen in isopentane over liquid nitrogen and embedded in OTC. I have
tried cutting at different temperatures, from -18C to -30C, with my
samples equilibrating in the machine at least an hour before I start.
I have seen protocols in which the tissue is embedded in OTC before
freezing, and also infusing the tissue with a mixture of sucrose and
OTC before embedding. Does anyone have any preference for these
methods?
I would really appreciate any advice!
Thank you!
Joanne
Curie Institute, Paris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail communication and any attachments may contain confidential and
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If you are not the intended recipient, you are hereby notified that you have
received this communication in error and that any review, disclosure,
dissemination, distribution or copying of it or its contents is prohibited.
As required by federal and state laws, you need to hold this information as
privileged and confidential. If you have received this communication in
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------------------------------
Message: 27
Date: Thu, 29 Sep 2005 11:23:33 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] Moh's Histotech salary range
To: "Snyder, Wendy" <SnyderW <@t> uhcwv.org>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF5324D6E5.82DFD116-ON8625708B.005A0439 <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
$150K per year, and I'll take the job.
"Snyder, Wendy" <SnyderW <@t> uhcwv.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
09/29/2005 10:37 AM
To: histonet <@t> lists.utsouthwestern.edu
cc:
Subject: [Histonet] Moh's Histotech salary range
My hospital is in the process of starting a Moh's Surgery program. We
will
be hiring an experienced Moh's histotech, HT(ASCP) soon. I know the
demand
for such a position is high. Could anyone give me an idea of the salary
range that a histotech gets paid for doing Moh's frozen sections? I would
really appreciate it.
Wendy Snyder HT(SCP)
United Hospital Center
Carksburg, WV 26301
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 22, Issue 38
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