[Histonet] Dissolving Alizarin complexon

[Gayle Callis]

Have you tried a larger pore size, 0.45 um.or larger.  One could always get 
the big particles first, then refilter again through smaller pore size if 
you felt it necessary.  I have had to do this with normal serums before 
when 0.22um clogs up.

Possibly, you could try centrifuging the stock solution, decant off the top 
what you need and microfilter that portion just to eliminate the biggest 
particles before you go to microfilter.

Just suggestions here


   At 01:46 AM 9/28/2005, you wrote:
>Hello,
>
>Is there anyone who is familiar with dissolving alizarin complexon for 
>bonelabeling?
>We inject this fluorochrome in our testanimals to monitor new 
>boneformation. Because of that we need to sterilize the solution. For this 
>we push the solution through a 0,2 um millipore-filter. The problem is 
>that the filter gets clogged very fast. We've tried prefiltering through a 
>paperfilter, but that doesn't solve the problem. Does anyone have a a 
>solution for this?
>
>Greetings,
>
>Léon Driessen,
>Orthopaedic Research Lab
>UMCN St. Radboud, Nijmegen,
>The Netherlands
>
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)