[Histonet] What's mellitin?

Tue Sep 27 14:13:27 CDT 2005

I never heard of mellitin. If you mean melanin, there is a bleach
method to remove it and compare to unbleached h&e slides and there is a
Fontana Masson which is a melanin silver stain.
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Today's Topics:

   1. lab design (Melissa Jans)
   2. RE: lab design (Jim Staruk)
   3. unsubscribe (Anne Van Binsbergen)
   4. unsubscribe (Anne Van Binsbergen)
   5. RE: lab design (Bartlett, Jeanine)
   6. Mellitin (TLCLab)

----------------------------------------------------------------------

Message: 1
Date: Sat, 24 Sep 2005 11:08:36 -0700 (PDT)
From: Melissa Jans <histomjans <@t> yahoo.com>
Subject: [Histonet] lab design
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050924180837.32315.qmail <@t> web54709.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I hope this email finds everyone well,

I have the opportunity to do some redesigning of our laboratory space. 
I would like some feedback from some of the larger labs as to whether
they have a separate room for their tissue processors and/or flammable
storage.  If so, how is it separate from the main laboratory working
space...wall, door, both?
Any feedback would be appreciated.
Melissa Jans
University of Iowa Hospitals and Clinics

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------------------------------

Message: 2
Date: Sat, 24 Sep 2005 20:59:22 -0400
From: "Jim Staruk" <jstaruk <@t> masshistology.com>
Subject: RE: [Histonet] lab design
To: "'Melissa Jans'" <histomjans <@t> yahoo.com>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0INC001DZLIAFRF3 <@t> vms046.mailsrvcs.net>
Content-Type: text/plain; charset=us-ascii

This is exactly what I did when I designed our new facilities.  I built
a
flame-resistant room which is well-ventilated directly to the outside. 
The
ventilation unit is adjustable for varying amounts of cubic feet per
minute
air exchange.  This unit is always on.  There is a special cabinet for
flammable chemicals, acid-resistant bench tops for the tissue
processors and
multiple, adjustable shelves for slide drying in this room.  The solid
wood
passage door is always closed.  I also built a fume hood directly on
the
other side of one of these walls and placed a blower unit (from an
old,
charcoal bench-top fume hood) through the wall.  This is where we
coverslip.
When the blower is on, it sucks away all of the xylene-laden air into
the
vented room which, in turn, is vented directly to the outside.  There
is
never any xylene fumes in the lab.  On our main homepage, there's a
link to
photos of our new lab.  Here, you will see the ventilated room as well
as
the coverslipping fume hood on the other side of the ventilated room.

Jim

______________________
    Jim Staruk
Mass Histology Service
www.masshistology.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Melissa
Jans
Sent: Saturday, September 24, 2005 2:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] lab design

I hope this email finds everyone well,

I have the opportunity to do some redesigning of our laboratory space. 
I
would like some feedback from some of the larger labs as to whether
they
have a separate room for their tissue processors and/or flammable
storage.
If so, how is it separate from the main laboratory working
space...wall,
door, both?
Any feedback would be appreciated.
Melissa Jans
University of Iowa Hospitals and Clinics

__________________________________________________
Do You Yahoo!?
Tired of spam?  Yahoo! Mail has the best spam protection around 
http://mail.yahoo.com 
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------------------------------

Message: 3
Date: Sun, 25 Sep 2005 14:57:20 +0400
From: "Anne Van Binsbergen" <vanann702 <@t> skmc.gov.ae>
Subject: [Histonet] unsubscribe
To: <histonet <@t> lists.utsouthwestern.edu>,
    <histonet <@t> pathology.swmed.edu>
Message-ID:
    <0C44F1AAEE47D54DA4210A60AB206F5E0445CC08 <@t> SKMCEMAIL.skmc.gov.ae>
Content-Type: text/plain;    charset="us-ascii"

------------------------------

Message: 4
Date: Sun, 25 Sep 2005 14:57:20 +0400
From: "Anne Van Binsbergen" <vanann702 <@t> skmc.gov.ae>
Subject: [Histonet] unsubscribe
To: <histonet <@t> lists.utsouthwestern.edu>,
    <histonet <@t> pathology.swmed.edu>
Message-ID:
    <0C44F1AAEE47D54DA4210A60AB206F5E0445CC08 <@t> SKMCEMAIL.skmc.gov.ae>
Content-Type: text/plain;    charset="us-ascii"

------------------------------

Message: 5
Date: Sun, 25 Sep 2005 08:22:08 -0400
From: "Bartlett, Jeanine" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] lab design
To: "Melissa Jans" <histomjans <@t> yahoo.com>,
    <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
    <CB857F6460D42E4AAEA195054A25406C0ACC2F7A <@t> m-ncid-2.ncid.cdc.gov>
Content-Type: text/plain;    charset="utf-8"

Hi Jan:

Currently we have a separate room containing our tissue processors and
embedding centers.  We also have a large flammable storage cabinet in
this room.  We have a separate room with our alcohol/solvent recycler
and there is a large flammable storage cabinet in there as well.  We
have 2 smaller flammable cabinets in routine lab space as well.

We are moving into a brand-new lab building this fall.  In the new lab
we also will have separate rooms; one for the processors and embedding
cetners and another for recycling.  I do not think there is flammable
storage in the new processing room (it's pretty small) but there is in
the recycling room.  I believe there will be at least one under-counter
flammable storage in a main lab space as well.

Jeanine Bartlett
CDC, Atlanta

    -----Original Message----- 
    From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Melissa Jans 
    Sent: Sat 9/24/2005 2:08 PM 
    To: histonet <@t> lists.utsouthwestern.edu 
    Cc: 
    Subject: [Histonet] lab design

    I hope this email finds everyone well,

    I have the opportunity to do some redesigning of our laboratory
space.  I would like some feedback from some of the larger labs as to
whether they have a separate room for their tissue processors and/or
flammable storage.  If so, how is it separate from the main laboratory
working space...wall, door, both?
    Any feedback would be appreciated.
    Melissa Jans
    University of Iowa Hospitals and Clinics

    __________________________________________________
    Do You Yahoo!?
    Tired of spam?  Yahoo! Mail has the best spam protection around
    http://mail.yahoo.com
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    Histonet mailing list
    Histonet <@t> lists.utsouthwestern.edu
    http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Sun, 25 Sep 2005 17:03:01 +0200
From: TLCLab <tlclab <@t> free.fr>
Subject: [Histonet] Mellitin
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4336BC25.9050801 <@t> free.fr>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hello,

Is there any - strict - histochemical method to caracterise mellitin 
(meaning not an immunohistochemical method) ?

Regards,

Florent.

------------------------------

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End of Histonet Digest, Vol 22, Issue 31
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