[Histonet] Iron stain

[Webb, Dorothy L]

I am having problems with the iron stain on bone marrow cores.  We
routinely run our irons on the Ventana Nexus, but, recently have had
problems with the core biopsies picking up the "blue" staining in the
histiocytes and other areas, making it hard for th pathologist to be
confident in the diagnosis.  Any suggestions as to why this is
happening?  I have tried running them down to sterile water and running
the stain by hand using Mallory's Modification for iron and I am still
seeing the staining being picked up in areas that should not be
staining.  HELP , and thanks!!
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