[Histonet] querry

[Vinnie Della Speranza]

Antigen retrieval is undertaken to "un-do" the masking of tissue antigens that results from formalin (formaldehyde) fixation.

fresh frozen tissues would generally not require this sort of pre-treatment.. unfixed tissues will not be resilient enough to withstand the high heat or harsh effects of extreme pH or proteolysis (depending upon the antigen retrieval method you use), hence you will see the sections come off the slides or look "chewed up"

are you certain that retrieval pre-treatment is necessary in fresh frozen, unfixed tissues, for the antigens you are studying ?

also, in general, thicker sections often have a greater tendency to lift off the slides.

Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue  Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974
>>> "Arvind" <arvind <@t> nbrc.res.in> 09/27/05 04:23 AM >>>
can any one please tell me how to do antigen retrieval on soft tissue, what 
i do is i took cryosections on slide for human fetal brain  tissue age upto 
7 months (prenatal) sections are 40 micron thick, when i do HIER these 
sections are comming off the slides at some points, i use gelatin  subbed 
slides for this  please guide what to do in detail


thanking in advance

Arvind Singh Pundir

National Brain Research Centre
gurgaon, Haryana, INDIA
arvind <@t> nbrc.res.in


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