[Histonet] Low temp Antigen Retrieval for Bone Sections

[Kim Merriam]

Hello All,
 
I will be performing some IHC on mouse femurs (about 12 or so different antibodies).  In the past, I have had a lot of trouble keeping these sections on the slides during HIER.  I did a quick search on the archives and there were lots of suggestions, but nothing definitive.
 
I was reading an article about "low temp AR", overnight at 60C in TEG buffer, pH 9.0. Has anyone tried this method?  Also - what does TEG buffer stand for?
 
Any help would be greatly appreciated.
 
Kim


Kim Merriam
Novartis
Cambridge, MA
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