[Histonet] antigen retrieval for IHC

[Maria Mejia]

Hello Everyone,

Here's the information:

"Antigen Retrieval IHC: Past, Present & Future" by Shan-Rong Shi,
Richard J. Cote & Clive R. Taylor is in the Journal of Histochemistry & 
Cytochemistry,
Vol. 45, 327-344, 1997

Puchtler H., Meloan S.N. (1985) "On the chemistry of formaldehyde 
fixation & its
effects on IHC reactions. Histochemistry 82: 201-204

Yours

Maria Bartola Mejia
Smith-Kettlewell Eye Research Institute
San Francisco, CA 94115
Email: maria <@t> ski.org
Phone: (415)-345-2185

John Kiernan wrote:

>Maria, can you give chapter and verse for the Shi/Taylor
>and Puchrler/Meloan papers? Most papers with Shi and Taylor
>among the authors are about high temperature antigen retrieval
>(boiling water, with pH optima for various antigens).
> 
>Holde Puchtler and Susan Meloan published many imortant papers
>about staining ad histochemistry in the 1970s=1980s. Susan M 
>was a histonetter in the 1990s. 
>
>*****************
> 
>Maria Mejia wrote:
>  
>
>>Hello,
>>
>>I believe it was sometime in June of this year that there was a
>>discussion on the
>>histonet regarding "the simplest form" of reversing the effects for formalin
>>fixation on tissue was to wash the tissue well (before) processing.
>>
>>Well, I just read the article "Antigen retrieval IHC: Past, Present, &
>>Future by
>>Shan-Rong Shi, richard J. Cote & Clive R. Taylor (can google this
>>article). It's
>>a very interesting! Anyway, the article has a section titled non-heating
>>AR method stating (this) same simple method by (Puchtler & Meloan). It also
>>goes on to say that Elias JM (1990) adopted this method routinely by storing
>>deparaffinized in fresh changes of 10% sucrose/PBS @ 4C overnight (before)
>>IHC.
>>
>>My questions are, does anyone know or tried this method used by Elias?
>>And, can anyone explain the mechanism of 10% sucrose/PBS play in this
>>AR method?
>>
>>Just curious!
>>
>>Maria Bartola Mejia
>>Smith-Kettlewell Eye Research Institute
>>San Francisco, CA 94115
>>Email: maria <@t> ski.org
>>Phone: (415)-345-2185
>>
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>>