[Histonet] RE: Histonet Digest, Vol 22, Issue 27
Peterson, Dan
1dpeterson <@t> meriter.com
Thu Sep 22 12:41:12 CDT 2005
We've got about a dozen different types of plants of various shapes and
sizes, even a rubber tree. They thrive very well on the fumes. That and
the fish water from our 50 gal. aquarium................
Daniel R Peterson HT(ASCP)
Histopathology Section Head
General Medical Laboratories
(608)-267-6557
1dpeterson <@t> meriter.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, September 22, 2005 12:03
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 22, Issue 27
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Today's Topics:
1. RE: CJD (Malam Jacqueline)
2. re: Fluorescent Illumination Systems (Carl Hobbs)
3. RE: Brain tissue frozen section (Johnson, Teri)
4. Mitochondria fluorescent marker (Gayle Callis)
5. haemoxygenase I or II (Edwards, R.E.)
6. Plants in lab (Cindy DuBois)
7. RE: Plants in lab (Marshall Terry Dr, Consultant
Histopathologist)
----------------------------------------------------------------------
Message: 1
Date: Thu, 22 Sep 2005 08:33:40 +0100
From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
Subject: [Histonet] RE: CJD
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <B75B29D97DE3E84ABC2276497D44C33B08160031 <@t> rlixch>
Content-Type: text/plain
Try
CJD Surveillance Unit
Old Pharmacy Building
Western General Hospital
Crewe Road
Edinburgh EH4 2XU
Scotland
Tel: 0131 332 2117
Fax: 0131 343 1404
Jacqui
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Message: 2
Date: Thu, 22 Sep 2005 09:03:57 +0100
From: "Carl Hobbs" <carl.hobbs <@t> kcl.ac.uk>
Subject: [Histonet] re: Fluorescent Illumination Systems
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201c5bf4c$2ed9c2d0$112b5c9f <@t> Carlos>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
I recently upgraded my three Axioskops/verts from 50W mercury to EXfo's
XCite 100W lamps. I have no regrets and highly recommend them: we use
DAPI,
Hoechst, Alexas 488 and 594 routinely. To me, the 100W mercury vapour
lamps
still have the"edge" over the Xcite lamps( I have one as well) in
brightness, tho. But, given the ease of use, the longevity of the lamps
and
the evenness of the illumination field I would recommend the Xcite
lamps.
NB: they create fan- noise, tho! Particularly the older ones; their new
models are much quieter Test one out, anyway.
------------------------------
Message: 3
Date: Thu, 22 Sep 2005 07:14:46 -0500
From: "Johnson, Teri" <TJJ <@t> Stowers-Institute.org>
Subject: [Histonet] RE: Brain tissue frozen section
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.0.1127408401.4944.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="iso-8859-1"
><snip>< Then we put the tissue (rat or
mouse brain) into 30% sucrose in 0.1 M phosphate buffer (PB) and leave
it for at least overnight (you don't say how long you leave it) or until
the tissue floats. ><snip><
That should read until the tissue sinks. :)
It's much easier to section this material if you have a cryostat that
has a temperature control on the specimen holder. I will echo the
suggestion that you try different freezing media, although we have never
had any trouble with the OCT compound. We have only done a couple
rodent brain frozen sections though.
If your section is laying flat on your blade/blade holder before picking
up, then part of the problem is in your mounting technique. I recommend
starting the pickup at one end of your tissue (at the edge closest to
you towards the bottom of the slide) and then learn to move the slide
slightly towards you as you lower it down to the blade. If you just
plop the slide down flat on your section, you will introduce wrinkles
and bubbles in your section.
Admittedly, I've never totally perfected whole brain sections and am
awed by those publications that show flawless sections (but wonder how
many it took to get that look!).
Good luck and let us know what works for you!
Teri Johnson
Stowers Institute for Medical Research
Kansas City, MO
------------------------------
Message: 4
Date: Thu, 22 Sep 2005 09:54:17 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Mitochondria fluorescent marker
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050922095329.01b4b7f8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Check with Molecular Probes on their website.
Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)
------------------------------
Message: 5
Date: Thu, 22 Sep 2005 17:12:00 +0100
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] haemoxygenase I or II
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC88BEDFD1FC3F468D0376A7C75465F705C755FA <@t> Saffron.cfs.le.ac.uk>
Content-Type: text/plain; charset="iso-8859-1"
Wanted antibodies to the above that work on mouse paraffin
processed tissues...thanks
Richard Edwards
MRC TOXICOLOGY UNIT
LEICESTER...U.K.....
------------------------------
Message: 6
Date: Thu, 22 Sep 2005 09:50:42 -0700 (PDT)
From: Cindy DuBois <dpahisto <@t> yahoo.com>
Subject: [Histonet] Plants in lab
To: tmmrosla <@t> healtheast.org
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20050922165043.65335.qmail <@t> web33413.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
We have several plants in our lab. African Violets seem to do extremely
well and bloom all year round. It adds some nice color to our
environment.
Cindy DuBois
Delta Pathology Assoc.
Stockton CA
---------------------------------
Yahoo! for Good
Click here to donate to the Hurricane Katrina relief effort.
------------------------------
Message: 7
Date: Thu, 22 Sep 2005 17:57:39 +0100
From: "Marshall Terry Dr, Consultant Histopathologist"
<Terry.Marshall <@t> rothgen.nhs.uk>
Subject: RE: [Histonet] Plants in lab
To: "Cindy DuBois" <dpahisto <@t> yahoo.com>,
<tmmrosla <@t> healtheast.org>
Cc: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<FE2DB935F8BBB546B8A1BBF3459C5A1F06432C9C <@t> LIL.xRothGen.nhs.uk>
Content-Type: text/plain; charset="iso-8859-1"
I'm all for plants in he lab. (or anywhere).
A Norfolk pine seems a tad extravagant. I've seen them about 20 metres
tall!
Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
Consultant Pathologist
Rotherham General Hospital
South Yorkshire
England
terry.marshall <@t> rothgen.nhs.uk
-----Original Message-----
From: Cindy DuBois [mailto:dpahisto <@t> yahoo.com]
Sent: 22 September 2005 17:51
To: tmmrosla <@t> healtheast.org
Cc: Histonet
Subject: [Histonet] Plants in lab
We have several plants in our lab. African Violets seem to do extremely
well and bloom all year round. It adds some nice color to our
environment.
Cindy DuBois
Delta Pathology Assoc.
Stockton CA
---------------------------------
Yahoo! for Good
Click here to donate to the Hurricane Katrina relief effort.
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