[Histonet] frozen section trichrome

[Steven Coakley]

I tried a gomori's trichrome on 2 FS tissues this morning,   rat muscle and rat liver.  The liver was exceptable although the smooth muscle was much more purlpe then on paraffin sections.  The muscle was opposite what it should have been, with green muscle and red collagen.
Heres the procedure I followed:  10 min in NBF, 5 min in Gills3, 15 min gomoris, rinse in 0.5% acetic water.
 
Steve

		
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