[Histonet] Bielschowsky Staining

[John A. Kiernan]

Silver methods (if they are working properly)
stain the axons, not the myelin sheaths. 
Unmyelinated and myelinated axons are shown. The
mechanism has not been thoroughly studied for
Bielschowsky-type methods, but in technically
simpler procedures such as Bodian's protargol 
it is known that the substrate of staining is 
a neurofilament protein within the axoplasm
(Gambetti et al. 1981 Science 213:1521-1522).
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Emily Jane Wiesner-Camm wrote:
> 
> Hello All.
> I have been staining some young rat brain sections with Bielschowsky
> stain, and I was wondering if anyone could tell me if the silver if
> deposited on only fully myelinated axons.
> Thank you in advance.
> Emily
> **
> 
> **
> 
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