[Histonet] RE: Histonet Digest, Vol 22, Issue 22: DAB disposal.

Tue Sep 20 07:16:25 CDT 2005

Annette:
The practice of treating DAB with bleach is not recommended as the final product is unkown.
According with Lunn & Sansone (1990) DAB can be destroyed as follows:
1- prepare a 0.2 M aqueous solution of potassium permanganate (31.6 g/litre).
2- prepare a2.0 M solution of sulfuric acid (112 ml of conc. acid/litre).
3- dilute the DAB in a way that its final concentration does not excede 0.9 mg/ml; you will have to do these calculations according with the DAB solution you use).
4 To EACH 10 ml of DAB solution add 5 ml of solution 1 and 5 ml of solution 2.
5- Allow the mixture for at least 10 hours. Now the solution will be NON mutagenic.
6- Add ascorbic acid (powder) until all color disappears).
7- Neutralize solution 6 with sodium bicarbonate (test with pH paper to pH=7).
8- Discard the solution down the drain PROVIDED that local water authorities have given their approval (which is something much more difficult that following the above procedure).
Silver nitrata is a "horse of a different color" being a metal (not very heavy, but a metal nevertheless). I advise you to talk with the personnel in Radiology because they deal with this product and recover the silver.
Rene J. Buesa

"Featherstone, Annette" <AFeatherstone <@t> KaleidaHealth.Org> wrote:
I would like to know how everyone is disposing of the ammonium-silver
nitrate waste and DAB. Any and all responses would be of great help.
Thanks
Annette Featherstone HT/MLT
Supervisor Anatomic Pathology
Kaleida Health
Buffalo General Hospital
100 High St
Buffalo NY 14203
716-859-2625

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, September 19, 2005 13:01
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 22, Issue 22

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Today's Topics:

1. HT student (pruegg <@t> ihctech.net)
2. RE: HT student (Lee & Peggy Wenk)
3. RE: New ASCP Classification and You (Lee & Peggy Wenk)
4. training in muscle biopsies handling (Saeed Al-shieban)
5. RE: HT student (Horn, Hazel V)
6. CAP Regulation (bliven.laura <@t> marshfieldclinic.org)
7. Santa Cruz CD31 (Jackie M O'Connor)
8. OCT4 antibody for immunohistochemistry. (Muhammad Tahseen)
9. bcl-2 and bcl-6 for Ventana users (Sebree Linda A.)
10. RE: New ASCP Classification and You (Patsy Ruegg)

----------------------------------------------------------------------

Message: 1
Date: Sun, 18 Sep 2005 12:59:41 -0600
From: pruegg <@t> ihctech.net
Subject: [Histonet] HT student
To: 
Message-ID: <200509181859.j8IIxtjW076484 <@t> pro12.abac.com>
Content-Type: text/plain; charset="us-ascii"

I am OJT a college student working towards HT certification, I presume. She
is a Jr. in college and taking all the required courses to sit for the
HT/HTL exam. She works in my lab for an hourly wage while attending school.
I fund a portion of her tuition costs at a local University. Is it
appropriate for me to ask this person to give me a commitment to pursue HT
certification when she becomes eligible? Is it also appropriate for me to
ask for a commitment from this person to work in my lab after she is
certified, at the going salary rate, of course?

Patsy

Patsy Ruegg, HT(ASCP)QIHC

IHCtech, LLC

Fitzsimmons BioScience Park

12635 Montview Blvd. Suite 216

Aurora, CO 80010

P-720-859-4060

F-720-859-4110

wk email pruegg <@t> ihctech.net

website www.ihctech.net

------------------------------

Message: 2
Date: Sun, 18 Sep 2005 18:37:57 -0400
From: "Lee & Peggy Wenk" 
Subject: RE: [Histonet] HT student
To: 
, 
Message-ID: <1085039814-324235194 <@t> pathology.swmed.edu>
Content-Type: text/plain; charset="us-ascii"

At our hospital lab, anyone we hire who is registry eligible has 1 1/2 years
in which to take and pass their exam (HT/HTL/CT/QIHC, etc). It is
stated/written when they are hired. If they do not take AND pass within that
time period, they are let go. We made it for 1.5 years, as that gives them
several attempts, in case they do fail. Everyone has taken and passed. Plus,
they know that once they pass the exam, they get bumped up to the next pay
level. Double incentive to take the exam early and pass it.

As for the commitment, I know of some labs that have offered my students to
pay sign-on bonuses, moving expenses, sometimes even out-right loans, with
the understanding (in writing, of course) that these are "free loans", if
the person comes to work for them for 1-2 (or 3) years. If the student
leaves before the 1-2-3 years, they must repay the loans - either the entire
amount, or the percentage left (in other words, if they agreed to work for 2
years (24 months), and they leave after 14 months, they had 10 months that
they did not fulfill the agreement, so have to pay back 10/24 of the "loan".

Just some thoughts.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48037

Lee & Peggy Wenk
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
pruegg <@t> ihctech.net
Sent: Sunday, September 18, 2005 3:00 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] HT student

I am OJT a college student working towards HT certification, I presume. She
is a Jr. in college and taking all the required courses to sit for the
HT/HTL exam. She works in my lab for an hourly wage while attending school.
I fund a portion of her tuition costs at a local University. Is it
appropriate for me to ask this person to give me a commitment to pursue HT
certification when she becomes eligible? Is it also appropriate for me to
ask for a commitment from this person to work in my lab after she is
certified, at the going salary rate, of course?

Patsy

Patsy Ruegg, HT(ASCP)QIHC

IHCtech, LLC

Fitzsimmons BioScience Park

12635 Montview Blvd. Suite 216

Aurora, CO 80010

P-720-859-4060

F-720-859-4110

wk email pruegg <@t> ihctech.net

website www.ihctech.net

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 3
Date: Sun, 18 Sep 2005 18:43:32 -0400
From: "Lee & Peggy Wenk" 
Subject: RE: [Histonet] New ASCP Classification and You
To: "'pam marcum'" ,

Message-ID:

Content-Type: text/plain; charset="us-ascii"

Pam - 

I'm curious. Who at NSH is saying this?

Renee Allegruci from the ASCP Board of Registry was at the NSH S/C (at the
ASCP BOR booth in the exhibit hall). She attended the Instructors of
Histotechnology meeting on Monday night. I asked her, in front of about a
dozen histology schools' program directors, if ASCP BOR was pursuing the lab
assistant exam route, and her answer was short - "No."

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pam marcum
Sent: Friday, September 16, 2005 2:18 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New ASCP Classification and You

Good Morning,

I have just returned from the NSH meeting. I was surprised to find out the
Histology Assistant or HA category recently presented to the NSH board and
refused with a letter is moving forward. No one had told the general
membership of NSH or ASCP or had it been explained to them in full.

It is important for those of us who have worked so hard to get the new the
education rules set in place to understand what this new registry will be
and how it will affect us as professionals. This is an aid position that is
OJT (on the job training) and will mean the pathologist, hospital or other
private histology laboratory will be able to avoid having more than
registered HT or HTL in the laboratory. The fully registered HT or HTL can
then be used as the supervisor or manager and responsible for all the HA's
work. This will aid in maintaining the lower pay scale we currently enjoy
and help prevent us raising our overall image as professionals. 

Please find out more about this and respond to the ASCP/BOR with comments
about this new category. The only way any of us will be heard is to let ASCP
know what we think and I hope many of you will join me in letting them know
we do not want to go back to the 1960s and 70s when we were all OJT and the
pay is still reflecting it. (By the way I was OJT and have worked hard to
improve my skills since then) We have worked hard for over thirty years to
begin to be recognized as professionals not just techs! DO NOT let this stop
us now so the pathologists at ASCP and AMA can continue to treat Histology
as minor part of the laboratory. Histology has changed and will continue to
make strides forward that require more education not less. 

The board of NSH did send a letter to the ASCP and tell them they would not
approve this category. It is a personal feeling however, I think the Board
of NSH should have notified the membership about this immediately, as a
letter is not sufficient to protect us or respond as members of both NSH and
ASCP. Again, write ASCP/BOR about your feelings if this important to you.

Second point Cathy Locallo put a motion forward at the House of Delegates to
request a Task Force to see if there is way to get histologists (HT and HTL)
working for the federal government recognized as professionals and here we
have a new way with the HA registry to keep us status quo. By the time the
task force is in place and moving we will be down a rung again.

Pamela Marcum 

(This is a reflection of my opinion not my employer or any other person.)

Pam Marcum

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 4
Date: Mon, 19 Sep 2005 08:15:27 +0300
From: Saeed Al-shieban 
Subject: [Histonet] training in muscle biopsies handling
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset=ISO-8859-1

hi
I am a medical tech.
I am interested in handling of muscle biopsies.
any body can advise me where is the best place to take training in that for

3 months at USA or UK
thanks

------------------------------

Message: 5
Date: Mon, 19 Sep 2005 08:35:49 -0500
From: "Horn, Hazel V" 
Subject: RE: [Histonet] HT student
To: pruegg <@t> ihctech.net, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C63038BDE21 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset=us-ascii

Just my thoughts but you should have required that of her before she
started attending school with you footing part of the bill. I think it
would be inappropriate to ask/demand it now. 

Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital
800 Marshall Mail Slot 820
Little Rock, AR 72202

phone- 501.364.4240
fax- 501.364.3912

visit us on the web at: www.archildrens.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lee &
Peggy Wenk
Sent: Sunday, September 18, 2005 5:38 PM
To: pruegg <@t> ihctech.net; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] HT student

At our hospital lab, anyone we hire who is registry eligible has 1 1/2
years in which to take and pass their exam (HT/HTL/CT/QIHC, etc). It is
stated/written when they are hired. If they do not take AND pass within
that time period, they are let go. We made it for 1.5 years, as that
gives them several attempts, in case they do fail. Everyone has taken
and passed. Plus, they know that once they pass the exam, they get
bumped up to the next pay level. Double incentive to take the exam early
and pass it.

As for the commitment, I know of some labs that have offered my students
to pay sign-on bonuses, moving expenses, sometimes even out-right loans,
with the understanding (in writing, of course) that these are "free
loans", if the person comes to work for them for 1-2 (or 3) years. If
the student leaves before the 1-2-3 years, they must repay the loans -
either the entire amount, or the percentage left (in other words, if
they agreed to work for 2 years (24 months), and they leave after 14
months, they had 10 months that they did not fulfill the agreement, so
have to pay back 10/24 of the "loan".

Just some thoughts.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48037

Lee & Peggy Wenk
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
pruegg <@t> ihctech.net
Sent: Sunday, September 18, 2005 3:00 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] HT student

I am OJT a college student working towards HT certification, I presume.
She is a Jr. in college and taking all the required courses to sit for
the HT/HTL exam. She works in my lab for an hourly wage while attending
school.
I fund a portion of her tuition costs at a local University. Is it
appropriate for me to ask this person to give me a commitment to pursue
HT certification when she becomes eligible? Is it also appropriate for
me to ask for a commitment from this person to work in my lab after she
is certified, at the going salary rate, of course?

Patsy

Patsy Ruegg, HT(ASCP)QIHC

IHCtech, LLC

Fitzsimmons BioScience Park

12635 Montview Blvd. Suite 216

Aurora, CO 80010

P-720-859-4060

F-720-859-4110

wk email pruegg <@t> ihctech.net

website www.ihctech.net

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 6
Date: Mon, 19 Sep 2005 09:13:53 -0500
From: bliven.laura <@t> marshfieldclinic.org
Subject: [Histonet] CAP Regulation
To: 
Message-ID: <2904d01c5bd24$5d44a7e0$8e0110ac <@t> mfldclinframe.org>

The College of American Pathologists requires:
"For immunohistochemistry tests that provide independent
predictive/prognostic information, does the patient report include
information on specimen processing, the antibody clone, and the scoring
method used.?"

Anyone willing to share their example? I'd like to keep it sweet, simple,
and short, but have it look clean and easy to read. Please send by email or
fax, void of all personal info please.

Thanks,

Laura Bliven
Histology Lab/IHC
Marshfield Laboratories
1000 N. Oak Ave.
Marshfield, WI 54449
bliven.laura <@t> marshfieldclinic.org
fax#715-389-5353

------------------------------

Message: 7
Date: Mon, 19 Sep 2005 11:15:26 -0500
From: "Jackie M O'Connor" 
Subject: [Histonet] Santa Cruz CD31
To: histonet <@t> pathology.swmed.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Does anyone know if Santa Cruz ever worked out the issue with their CD31 
SC1506 antibody? Last I heard, their goat was sick.
Is it safe to order this antibody with a reasonable expectation that it 
will work?

Jackie O'

------------------------------

Message: 8
Date: Mon, 19 Sep 2005 21:22:10 +1200
From: "Muhammad Tahseen" 
Subject: [Histonet] OCT4 antibody for immunohistochemistry.
To: "HistoNet Server" 
Message-ID: <002401c5bcfb$9e1c5fc0$972bfea9 <@t> m7c0y4>
Content-Type: text/plain; charset="iso-8859-1"

Dear All,

Our lab is looking for OCT4 antibody for immunohistochemistry identification
of seminoma and embryonal carcinoma. Could you please provide us with the
necessary information so that we can order the antibody.

Thank you.

Muhammad Tahseen
Histology Supervisor
SKMCH & RC
Lahore Pakistan.

------------------------------

Message: 9
Date: Mon, 19 Sep 2005 11:34:33 -0500
From: "Sebree Linda A." 
Subject: [Histonet] bcl-2 and bcl-6 for Ventana users
To: "Histonet \(Histonet\)" 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello, 

I'm having some difficulty working up bcl-6 on our Ventana instruments
and the bcl-2 we've always used isn't as robust as I'd like either.
What clones and vendors are people using on their Ventana automated
stainers? We have a NexES, a BenchMark and a BenchMark XT.

Thanks for the info.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

------------------------------

Message: 10
Date: Mon, 19 Sep 2005 10:49:28 -0600
From: "Patsy Ruegg" 

Subject: RE: [Histonet] New ASCP Classification and You
To: "'Stephen Peters M.D.'" 
,

Message-ID: <200509191649.j8JGnNfj002085 <@t> chip.viawest.net>
Content-Type: text/plain; charset="us-ascii"

Before we all get bent out of shape about this we need to verify that ASCP
is really going forward with this classification or not. I am on the NSH
BOD and at no time did we have any discussions about this with the
assumption that ASCP is going forward with this. We need to ask Marilyn
Gamble the NSH ASCP representative to clarify.
Patsy 

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net

This email is confidential and intended solely for the use of the Person(s)
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stephen
Peters M.D.
Sent: Saturday, September 17, 2005 8:08 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New ASCP Classification and You

I think this is a step backwards for this field. By approving of such a
category they are sending the message this job is so easy you can just take
anyone off the street give them a few lessons and out will pop your insitu
hybridization. Your payscale will go backwards. 
I cannot imagine how anyone can understand what they are doing in this
rather complex field without a basic knowledge of the scientific and
technical principals you are using every day. Between the myriad of
histochemical stains you need to know, you have now added
immunohistochemistry and molecular techniques. These are very sophisticated
scientific procedures. Think about the minutia of details and experience as
well as technical information about your microtomes that is required just to
get a nice clean unshattered ribbon of a GI biopsy. Sure, you can show
someone the technical maneuvers in your free time but they will not reach
nearly the same level of quality without a knowledge of the principals. As
the guy reading the slides I would rather see your field require formal
education and certification at all levels. I have no problem with OJ T's
performing clerk duties, filing and such, but it is a bit scary to think of
letting one of these loose on my biopsies or cover immunos becase every one
is on vacation. 

My suggestion for these HA's is to designate an approved set of duties, much
like doctors are given privileges for certain procedures and not others when

=== message truncated ===

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