[Histonet] question about the embryonic brain fixation

[Ali Moussavi Nik]

Dear All

I would like to section some mouse embryonic brain (18.5 days) with
vibrotom.

I will do some Immunocytochemistry on them .I noticed that perfusion of the
mother is the best way for fixation of the embryo's brain. but which
fixative makes the lowest background?

Could some one give me a good protocol for that.

Thanks in advance.

Ali

 

 

 

 

  Ali Moussavi Nik 

  D.V.M, PhD student

  Department of Cell and Molecular Biology,Lundburg lab,

 Gothenburg,Sweden