[Histonet] sudan black/bleaching

[Helen Ilsley]

-- 
Hi

I wonder if any one can help me.
I am using sudan black to suppress my backgound autofluorescence but 
when I want to photograph my section I seem to be getting 
photobleaching especially at high mags. The image under the 
microscope  blurs and when I move away from the area I am left with a 
bleached, blurry ring.
Is this due to the sudan black?

Has anyone ever had this sort of problem before.
I would really appreciate any help.


Thanks

Helen Ilsley

Chris Barnard Division of Cardiothoracic Surgery

Cape Heart Centre

University of Cape Town Medical School

Cape Town, South Africa