[Histonet] Re: Calcium staining

[Malam Jacqueline]

Von Kossa is one we have used in the past- 
Reagents:
0.1M citrate buffer pH 4.5: 0.2M disodium hydrogen phosphate - 9.09ml
0.1M citric acid - 10.91ml

5% silver nitrate

5% sodium thiosulphate 

Method:
Take 2 sections to water
Immerse 1 in citrate buffer - 20 mins for neg control
Wash both slides and a coplin jar well in dist water
Put slides in coplin jar and fill with 5% silver nitrate
Expose to bright sunlight-10 to 20 mins or (if you are in England) a 60 watt
bulb at range of 4-5 inches - 30-60 mins
Wash both slides well in dist water
Treat with 5% sodium thiosulphate - 2-3 mins
Counterstain
Dehydrate, clear and mount

Alizarin red is also a good method if you have the dye.


Jacqui Malam
Royal Infirmary
Lancaster
England



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