[Histonet] Giemsa bits...

[Gagnon, Eric]

Hello Histonetters,
 
We have an intermittent problem with our Giemsa stain for helicobacter. ( We are using EMD Giemsa stain catalogue # R03055/74.)  The intermittent problem is a diamond-shaped deposit over the slide which is visible microscopically upon removal of slides from the warm solution.  Not sure if it is reagent-related or not. We have tried to standardize all other factors i.e. pH, mechanical/technique variations among techs, staining time, filtering etc.  Just wondering if anyone else out there has had the same problem.  So far, pathologists are not complaining, as they can see around it, but it would be nice to eradicate it!!
 
Thanks in advance,
Eric