Fwd: Re: [Histonet] endogenous peroxidases in paraffin

[LaCinda Burchell]

>>> LaCinda Burchell 09/13/05 9:08 AM >>>
Hello, and Good Morning;  I've never seen differences in frozen versus paraffin tissues.  Endogenous peroxidase is an important issue in bloody tissues (liver, spleen).  If you are working with extremely fragile specimens low in red blood cells, and you've done a side-by-side demonstration of blocked and un-blocked specimens you should be ok.  good luck! Cindy
>>> "Andrea T. Hooper" <anh2006 <@t> med.cornell.edu> 09/13/05 8:37 AM >>>
Does anyone have any thoughts on how much endogenous peroxidase 
remains in tissue after paraffin processing, I have experienced 
certainly less than frozen sections for sure, but still I want some 
stories from personal experience.

I am trying to decide whether I should try to exclude some glucose 
oxidase block on paraffin sections as it seems to be unnecessary in 
one of my protocols.

Thanks,
Andrea
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LaCinda Burchell, BA, AS, HT(ASCP)
University of Wisconsin-Madison, Medical School
Asthma and Allergy Research IHC Lab
600 Highland Ave.  CSC  K4/913
Madison, Wisconsin  53792

Phone: 608-262-3518
FAX:     608-263-3746