[Histonet] RE: distinguishing between mouse and human cells

[PALMER Jason (SVHM)]

We have used an anti human mitochondrial Ab to distinguish between human and mouse cells which worked quite nicely. Ours came from Calbiochem, although i am fairly sure they are no longer manufacturing it. There may be other companies producing a similar product though.

Jason

Jason Palmer
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
Australia
tel +61 3 9288 4018
fax +61 3 9416 0926
email: palmerj <@t> svhm.org.au




Message: 6
Date: Fri, 9 Sep 2005 13:00:45 -0700 (PDT)
From: Greg Lyness <greg_optimum <@t> yahoo.com>
Subject: [Histonet] distinguishing mouse vs. human cells
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050909200045.89785.qmail <@t> web33609.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dear histonetters,

We are working with xenograft models of human
pancreatic tumors implanted into the pancreas of nude
mice.  In order to prove that the tissue which is
growing is actual human tumor (and not mouse cells) we
want to stain for a specific antigen/marker to
distinguish between human and mouse cells.  Has anyone
ever immunostained for any such antigen/marker, and if
so, what did you use and from where did you obtain it?


Thanks in advance,

Greg Lyness
Research Scientist
Optimum Therapeutics
Columbus, OH
(614) 688-5885 

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