[Histonet] H&E

[Rogerson Kemlo (ELHT) Pathology]

You don't have an acid/ alcohol step? Either use a fresh progressive
haematoxylin step or introduce an acid/ alcohol step to remove the
'overstaining', then 'blue'.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Enniss,
Angel
Sent: 07 September 2005 14:35
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] H&E

I have been working on getting a new staining protocol or new
Hematoxyilin.
My supervisor does not like the blue  background and she thinks we can
do
better than Harris. I am doing a demo on a linear stainer. I want to try
lowering our times down to 30 seconds and using Harris with acid.
 
6 stations-xylene
2 stations-100%
1 station-95%
2 stations-running tap water
2 stations-Hematoxylin
3 stations-tap water
1 station-80%
1 station-Eosin
2 Station-95%
3 Station-100%
2 Stations-Xylene
 
Does anyone else have any better ideas for an automated stainer or
linear
stainer. If you could send protocols that would be useful.
 
Thanks so much.
 
Angel C. Enniss, HT (ASCP)
World Wide Safety Sciences AA
e-mail:  <mailto:angel.enniss <@t> pfizer.com> angel.enniss <@t> pfizer.com
Tel 734 622 3154
Fax 734 622 3866
 


LEGAL NOTICE
Unless expressly stated otherwise, this message is confidential and may
be privileged. It is intended for the addressee(s) only. Access to this
E-mail by anyone else is unauthorized. If you are not an addressee, any
disclosure or copying of the contents of this E-mail or any action taken
(or not taken) in reliance on it is unauthorized and may be unlawful. If
you are not an addressee, please inform the sender immediately.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet