[Histonet] H&E

[Enniss, Angel]

I have been working on getting a new staining protocol or new Hematoxyilin.
My supervisor does not like the blue  background and she thinks we can do
better than Harris. I am doing a demo on a linear stainer. I want to try
lowering our times down to 30 seconds and using Harris with acid.
 
6 stations-xylene
2 stations-100%
1 station-95%
2 stations-running tap water
2 stations-Hematoxylin
3 stations-tap water
1 station-80%
1 station-Eosin
2 Station-95%
3 Station-100%
2 Stations-Xylene
 
Does anyone else have any better ideas for an automated stainer or linear
stainer. If you could send protocols that would be useful.
 
Thanks so much.
 
Angel C. Enniss, HT (ASCP)
World Wide Safety Sciences AA
e-mail:  <mailto:angel.enniss <@t> pfizer.com> angel.enniss <@t> pfizer.com
Tel 734 622 3154
Fax 734 622 3866
 


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