[Histonet] anti VEGF on mouse tissue

[Patsy Ruegg]

I use monoclonal vegf from Santa Cruz, there is only one, at 1:200 with HIER
(I use steam for 20 min. with citrate buffer ph6), over night incubation of
the primary at 4dc and labelled polymer hrp detection for 60 min. at rt, DAB
10 min.
I have also found it necessary to use h202 with methanol to quench
endogenous peroxidase.
Oops, this is not going to work on you mouse tissue, guess you will have to
try one of the polyclonal vegf's or a ms on ms detection.  I use monoclonal
vegf on human, rabbit, sheep and rat tissue, not mouse.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
christelle.gerard <@t> novartis.com
Sent: Tuesday, September 06, 2005 6:07 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] anti VEGF on mouse tissue

Hello,

I already tested differents antibodies from Santa Cruz to detect VEGF on
mouse tissues fixed in formalin and embedded in paraffin. There was no
result.
Have you any experience in this IHC?
Thanks in advance for any informations, references or protocoles.

Christelle








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