[Histonet] (no subject)

[Lori Richey]

We fix all precut control tissue  slides in cold acetone for 5 
minutes,and allow them to air dry before storing them in the -70. 

Matt Prideaux wrote:

>Dear Histonetters,
>
>A colleague wishes to store cytospun cell preparations in the freezer before
>doing immunocytochemistry for CD31 and CD44. She would like to know whether
>it's better to fix the slides before freezing or after thawing them prior to
>staining. Also, which fixatives would you recommend? 
>
>Matt
>  
>