[Histonet] Immunofluorescence - CAP ?

Lori K. Richey lrichey <@t> u.washington.edu
Mon Oct 31 19:30:46 CST 2005

For IgA,M and G we use frozen tonsil.  Fibrinogen we use frozen placenta. If its C4d?  we use frozen kidney.

On Mon, 31 Oct 2005 bliven.laura <@t> marshfieldclinic.org wrote:

> A new CAP (College of American Pathologists) question has appeared on our checklist for the next inspection. The question asks, "Are appropriate positive and negative controls performed with each case tested using immunofluorescence?"  Sometimes positive controls not abundant and since we are working with only frozen tissue, does anyone have any helpful ideas? Other than past positive cases, is there any other positive tissue that we could use for IgA, IgM, IgG, C3, Fibrinogen, properdin and/or C4?
> Thanks much,
> Laura
> Laura Bliven
> Histology Lab/IHC
> Marshfield Laboratories
> 1000 N. Oak Ave.
> Marshfield, WI 54449
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